CAJ | 학술논문

目的检查肿瘤抑制蛋白FBW7在鳞状细胞癌（ OSCC）中的表达，初步探究FBW7表达与OS-CC细胞克隆形成能力的关系。方法收集19例OSCC患者的癌巢组织及对应癌旁组织，应用免疫组化染色与Real time RT-PCR技术检测FBW7在癌巢及对应癌旁组织中的表达情况。 Western blot 和Real time RT-PCR技术比较口腔鳞状细胞癌细胞系KV、CTST-1、CTST-2以及正常颊黏膜上皮细胞中FBW7蛋白和mRNA表达水平，分析FBW7表达与口腔鳞状细胞癌细胞系克隆形成能力的关系。结果免疫组化和Real time RT-PCR结果显示，FBW7蛋白及mRNA在对应癌旁组织中的表达水平明显高于癌巢组织（P＜0.05），West-ern blot及Real time RT-PCR结果显示，FBW7蛋白和mRNA在口腔鳞癌细胞系中的表达水平明显低于正常口腔黏膜上皮细胞（ P＜0.05）。克隆形成实验结果表明， FBW7表达较高的舌癌细胞株的克隆形成数目较少，克隆形成能力较弱。结论 FBW7在口腔鳞状细胞癌组织和细胞系中表达显著降低，与口腔癌细胞系的克隆形成能力呈负相关。
목적검사종류억제단백FBW7재린상세포암（ OSCC）중적표체，초보탐구FBW7표체여OS-CC세포극륭형성능력적관계。방법수집19례OSCC환자적암소조직급대응암방조직，응용면역조화염색여Real time RT-PCR기술검측FBW7재암소급대응암방조직중적표체정황。 Western blot 화Real time RT-PCR기술비교구강린상세포암세포계KV、CTST-1、CTST-2이급정상협점막상피세포중FBW7단백화mRNA표체수평，분석FBW7표체여구강린상세포암세포계극륭형성능력적관계。결과면역조화화Real time RT-PCR결과현시，FBW7단백급mRNA재대응암방조직중적표체수평명현고우암소조직（P＜0.05），West-ern blot급Real time RT-PCR결과현시，FBW7단백화mRNA재구강린암세포계중적표체수평명현저우정상구강점막상피세포（ P＜0.05）。극륭형성실험결과표명， FBW7표체교고적설암세포주적극륭형성수목교소，극륭형성능력교약。결론 FBW7재구강린상세포암조직화세포계중표체현저강저，여구강암세포계적극륭형성능력정부상관。
Objective To analyze the expression of FBW7 in oral squamous cell carcinoma (OSCC),and to preliminarily investigate the correlation of FBW 7( F-box and WD repeat domain-containing 7,FBW7) expression and colony formation capability of OSCC .Methods 19 cancerous tissues and adjacent noncancerous tissues were collected from those patients who had been diagnosed the primary OSCC during their surgeries .The expression levels of FBW7 protein and mRNA were examined using immunohistochemistry and real time RT-PCR to determine the prognostic significance .The correlation between FBW7 expression and colony formation capability in OSCC cell lines were investigated in vitro.Results We found that FBW7 expression was significantly higher in adjacent noncancerous tissues than that in the cancerous tissues though immunohistochemistry and real time RT -PCR ( P<0.05).Similarly,the expression of FBW7 protein and mRNA was also higher in normal epithelial cells than that in OSCC cell lines ( P<0.05 ) .There was a negative correlation between the expression of FBW 7 mRNA and colony formation capability in the soft agar colony formation assay ( P<0.05 ) .Conclusions The expression of FBW7 in OSCC tissue and cell lines is downregulated .The expressions of FBW 7 mRNA have a negative relation with the colony formation capability of OSCC cell lines .