CAJ | 학술논문

目的探讨血清和脑脊液中基质金属蛋白酶（ MMP）-9及其抑制剂金属蛋白酶组织抑制剂（ TIMP）-1与多发性硬化发病及疾病活动的相关性。方法本实验共分四组：多发性硬化组、神经系统炎性疾病（OIND）组、神经系统非炎性疾病（NIND）组和健康人群组。采用酶联免疫吸附试验（ELISA）检测血清MMP-9、血清和脑脊液中TIMP-1的表达水平；采用酶谱法检测脑脊液中MMP-9水平；以MRI检查为多发性硬化疾病活动性判断标准，分析比较多发性硬化活动组和非活动组MMP-9及TIMP-1水平。结果多发性硬化组血清MMP-9水平显著高于NIND组（ P＜0.05）和健康人群组（ P＜0.001），血清TIMP-1水平显著低于OIND组（ P＜0.001）、NIND组（ P＜0.001）和健康人群组（ P＜0.001）；多发性硬化组脑脊液中MMP-9水平高于NIND组（ P＜0.05），脑脊液中TIMP-1水平显著低于OIND组（ P＜0.05）；多发性硬化活动期患者血清MMP-9水平、MMP-9/TIMP-1比值分别显著高于非活动期（ P均＜0.05），活动期血清TIMP-1水平与非活动期无统计学差异。结论检测血清和脑脊液中MMP-9及TIMP-1水平对多发性硬化疾病活动性的监测有一定参考价值。
목적탐토혈청화뇌척액중기질금속단백매（ MMP）-9급기억제제금속단백매조직억제제（ TIMP）-1여다발성경화발병급질병활동적상관성。방법본실험공분사조：다발성경화조、신경계통염성질병（OIND）조、신경계통비염성질병（NIND）조화건강인군조。채용매련면역흡부시험（ELISA）검측혈청MMP-9、혈청화뇌척액중TIMP-1적표체수평；채용매보법검측뇌척액중MMP-9수평；이MRI검사위다발성경화질병활동성판단표준，분석비교다발성경화활동조화비활동조MMP-9급TIMP-1수평。결과다발성경화조혈청MMP-9수평현저고우NIND조（ P＜0.05）화건강인군조（ P＜0.001），혈청TIMP-1수평현저저우OIND조（ P＜0.001）、NIND조（ P＜0.001）화건강인군조（ P＜0.001）；다발성경화조뇌척액중MMP-9수평고우NIND조（ P＜0.05），뇌척액중TIMP-1수평현저저우OIND조（ P＜0.05）；다발성경화활동기환자혈청MMP-9수평、MMP-9/TIMP-1비치분별현저고우비활동기（ P균＜0.05），활동기혈청TIMP-1수평여비활동기무통계학차이。결론검측혈청화뇌척액중MMP-9급TIMP-1수평대다발성경화질병활동성적감측유일정삼고개치。
Objective To assess serum and cerebrospinal fluid ( CSF ) levels of matrix metalloproteinase-9 (MMP-9)and tissue inhibitors of metalloproteinase (TIMP)-1 of multiple sclerosis(MS)patients,and explore the association with disease active stage .Methods Four groups of subjects were tested:MS, other inflammatory neurological disorders(OIND),non-inflammatory neurological disorders(NIND)and health control(HC).MMP-9 of serum and TIMP-1of serum and CSF were measured by enzyme linked immunosorbent assay ( ELISA);The levels of MMP-9 of CSF were measured by gelatin-substrate zymography .Results Serum MMP-9 concentrations were higher in MS than in NIND and in HC(P<0.05,<0.001,respectively),Serum TIMP-1 concentrations were lower in MS than in NIND,in OIND and in HC(P<0.001,<0.001,<0.001,respectively).CSF mean levels of MMP-9 were more elevated in MS than in NIND ( P<0.05 ) , CSF mean levels of TIMP-1 were lower in MS than in OIND ( P<0.05 ) .Serum MMP-9 and MMP-9/TIMP-1 ratio mean levels were increased in MS patients with MRI disease activity compared to patients with non-active disease ( P <0.05 , <0.05 , respectively ) .The differences of serum mean concentrations of TIMP-1 between MS patients with MRI disease activity and patients with non-active disease did not reach statistical significance .Conclusion To investigate serum and CSF levels of MMP-9 and TIMP-1 is valuable in assessing disease activity of MS .