CAJ | 학술논문

目的：观察外周血CD4＋CD28-T细胞、CD4＋CD25＋T细胞亚群在2型糖尿病患者及大血管病变患者的变化，探讨其在2型糖尿病及大血管病变发病中的作用。方法：应用免疫荧光标记技术和流式细胞仪，检测36例初诊2型糖尿病（单纯DM组）患者和28例糖尿病并大血管病变患者、20例健康对照者的外周血T细胞亚群变化。结果：DM并大血管病变组、单纯DM 组 CD4＋CD28-T 细胞明显高于健康对照组，差异均有统计学意义（P<0.05）；而 CD4＋CD25＋T 细胞明显低于健康对照组（P<0.05）；DM并大血管病变组CD4＋CD28-T细胞高于单纯DM组（P<0.05），CD4＋CD25＋T细胞低于单纯DM组。结论：机体细胞免疫异常在2型糖尿病及大血管病变的发生、发展中发挥着一定的作用。
목적：관찰외주혈CD4＋CD28-T세포、CD4＋CD25＋T세포아군재2형당뇨병환자급대혈관병변환자적변화，탐토기재2형당뇨병급대혈관병변발병중적작용。방법：응용면역형광표기기술화류식세포의，검측36례초진2형당뇨병（단순DM조）환자화28례당뇨병병대혈관병변환자、20례건강대조자적외주혈T세포아군변화。결과：DM병대혈관병변조、단순DM 조 CD4＋CD28-T 세포명현고우건강대조조，차이균유통계학의의（P<0.05）；이 CD4＋CD25＋T 세포명현저우건강대조조（P<0.05）；DM병대혈관병변조CD4＋CD28-T세포고우단순DM조（P<0.05），CD4＋CD25＋T세포저우단순DM조。결론：궤체세포면역이상재2형당뇨병급대혈관병변적발생、발전중발휘착일정적작용。
Objective:To investigate the changes of CD4+CD28-T cells, CD4+CD25+T cells and their roles in the development of type 2 diabetes and its macrovascular complications. Methods: T lymphocyte subsets of periphera1 blood mononuclear cells (PBMCS) were determined with immunofluorescence staining and flow cytometry method from 36 patients with type 2 diabetes mellitus and 28 patients with macrovascular disease, 20 healthy controls. Results:The percentage of CD4+CD28-T cells increased while CD4+CD25+T cells were decreased in those with type 2 diabetes and macrovascular disease (P<0.05).Al1 of the above changes had significantly difference from those in control group (P<0.05). CD4+CD28-T cells in the group of macrovascular disease were also higher than those in type 2 diabetes and CD4+CD25+T cells were lower. Conclusion:Immune dysfunction exerts have certain effects on the pathogenesis and progression of type 2 diabetes mellitus and macrovascular disease.