检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2013年
19期
2546-2547,2549
,共3页
乙型肝炎表面抗原%酶联免疫吸附试验%两步法%影响因素
乙型肝炎錶麵抗原%酶聯免疫吸附試驗%兩步法%影響因素
을형간염표면항원%매련면역흡부시험%량보법%영향인소
HBsAg%ELISA%two-step detection%influencing factors
目的探讨酶联免疫吸附试验(ELISA )两步法检测乙型肝炎病毒表面抗原(HBsAg )的影响因素。方法分别在稀释液的量、洗板时机、孵育时间、显色时间等不同条件下对阳性混合血清、阴性混合血清和弱阳性对照血清进行检测。结果使用滴瓶滴加1滴稀释液、不加入稀释液、加入血清后温育时间缩短为45 min 、加入酶结合物后孵育时间缩短为15 min 、加入显色剂后显色时间缩短为15 min ,弱阳性结果较标准方法差异有统计学意义;加入样本孵育1 h 后洗板,阳性、弱阳性和阴性结果均较标准方法差异有统计学意义。结论 ELISA 条件的改变,就强阳性标本而言或许影响不大,但弱阳性标本的结果会被显著影响,尤其是处于临界值(灰区)的标本所受影响可能就更为明显。
目的探討酶聯免疫吸附試驗(ELISA )兩步法檢測乙型肝炎病毒錶麵抗原(HBsAg )的影響因素。方法分彆在稀釋液的量、洗闆時機、孵育時間、顯色時間等不同條件下對暘性混閤血清、陰性混閤血清和弱暘性對照血清進行檢測。結果使用滴瓶滴加1滴稀釋液、不加入稀釋液、加入血清後溫育時間縮短為45 min 、加入酶結閤物後孵育時間縮短為15 min 、加入顯色劑後顯色時間縮短為15 min ,弱暘性結果較標準方法差異有統計學意義;加入樣本孵育1 h 後洗闆,暘性、弱暘性和陰性結果均較標準方法差異有統計學意義。結論 ELISA 條件的改變,就彊暘性標本而言或許影響不大,但弱暘性標本的結果會被顯著影響,尤其是處于臨界值(灰區)的標本所受影響可能就更為明顯。
목적탐토매련면역흡부시험(ELISA )량보법검측을형간염병독표면항원(HBsAg )적영향인소。방법분별재희석액적량、세판시궤、부육시간、현색시간등불동조건하대양성혼합혈청、음성혼합혈청화약양성대조혈청진행검측。결과사용적병적가1적희석액、불가입희석액、가입혈청후온육시간축단위45 min 、가입매결합물후부육시간축단위15 min 、가입현색제후현색시간축단위15 min ,약양성결과교표준방법차이유통계학의의;가입양본부육1 h 후세판,양성、약양성화음성결과균교표준방법차이유통계학의의。결론 ELISA 조건적개변,취강양성표본이언혹허영향불대,단약양성표본적결과회피현저영향,우기시처우림계치(회구)적표본소수영향가능취경위명현。
Objective To discuss the influencing factors on two-step enzyme linked immunosorbent assay (ELISA) detection of hepatitis B virus surface antigen (HBsAg ) .Methods Positive ,negative and weakly positive mixed serum were detected under different dilution ,plate washing time ,incubation time and color time .Results With one drop or without dilute solution ,incubation time shortened respectively to 45 and 15 min after adding serum or en-zyme conjugates ,color time changed to 15 min with chromogenic agents ,the weakly positive result was statistically different with standard methods .The positive ,weakly positive and negative results were also statistically different with standard methods when plate was washed after incubation for 1 h .Conclusion The changes of ELISA condi-tions might have obvious effect on weakly positive samples especially those within critical value range (gray zone) , though the effect could be ignored in positive ones .