CAJ | 학술논문

目的：探讨二硫代氨基甲酸吡咯烷（PDTC）对大鼠心肌梗死后核转录因子κB（NF-κB）、基质金属蛋白酶-2（MMP-2）的表达及胶原重塑的影响。方法：制作大鼠心肌梗死模型，分为 PDTC干预（PD）组和心肌梗死对照（MI）组，另设假手术（SH）组，每组大鼠6只。PD组于术后24 h腹腔注射PDTC 80 mg · kg -1· d-1，连续给药28 d后行超声心动图检查评定心功能，处死动物，计算总胶原、Ⅰ型胶原、Ⅲ型胶原含量和梗死面积，实时荧光定量逆转录聚合酶链反应和免疫印迹法分别检测N F-κBp65及MMP-2的mRNA和蛋白质表达量。结果与SH组比较，MI组和 PD组的总胶原、Ⅰ型胶原、Ⅰ/Ⅲ胶原比值明显增大，NF-κBp65和MMP-2的mRNA及蛋白质表达量明显增加，差异均具有统计学意义（P＜0．01）。与MI组比较，PD组总胶原、Ⅰ型胶原、Ⅰ/Ⅲ胶原比值改变明显减轻，NF-κBp65和 MMP-2的 mRNA 及蛋白质表达量明显降低，差异有统计学意义（P＜0．01）。结论： PDTC在一定程度上能改善大鼠心肌梗死后心肌胶原重塑，其机制可能与抑制 NF-κB激活，下调基质金属蛋白酶（如MMP-2）表达有关。
목적：탐토이류대안기갑산필각완（PDTC）대대서심기경사후핵전록인자κB（NF-κB）、기질금속단백매-2（MMP-2）적표체급효원중소적영향。방법：제작대서심기경사모형，분위 PDTC간예（PD）조화심기경사대조（MI）조，령설가수술（SH）조，매조대서6지。PD조우술후24 h복강주사PDTC 80 mg · kg -1· d-1，련속급약28 d후행초성심동도검사평정심공능，처사동물，계산총효원、Ⅰ형효원、Ⅲ형효원함량화경사면적，실시형광정량역전록취합매련반응화면역인적법분별검측N F-κBp65급MMP-2적mRNA화단백질표체량。결과여SH조비교，MI조화 PD조적총효원、Ⅰ형효원、Ⅰ/Ⅲ효원비치명현증대，NF-κBp65화MMP-2적mRNA급단백질표체량명현증가，차이균구유통계학의의（P＜0．01）。여MI조비교，PD조총효원、Ⅰ형효원、Ⅰ/Ⅲ효원비치개변명현감경，NF-κBp65화 MMP-2적 mRNA 급단백질표체량명현강저，차이유통계학의의（P＜0．01）。결론： PDTC재일정정도상능개선대서심기경사후심기효원중소，기궤제가능여억제 NF-κB격활，하조기질금속단백매（여MMP-2）표체유관。
Objective Toinvestigatetheeffectsofpyrrolidinedithiocarbamate(PDTC)onexpressionofNF-κB,MMP-2andleft ventricular collagen remodeling following acute myocardial infarction in rats .Methods The myocardial infarction model in rat was induced by ligation of left anterior descending coronary artery .12 adult Sprague-Dawley rats survived 24 for h after acute myocardial infarction were randomly divided into the myocardial infarction (MI) group and the PDTC-treated(PD) group .Six rats were desig-nated as sham-operated group(SH group) .The PD group was intraperitoneally injected with PDTC (80 mg · kg -1 · d-1 ) for 28 d , the MI group and SH group were given normal saline as control .On 28 d ,the cardiac function of left ventricle was measured by ech-ocardiography .The infarct size was evaluated .The total collgen ,typeⅠcollgen ,typeⅢcollgen ,and Ⅰ /Ⅲ collgen ratio were quanti-fied histomorphometry .The mRNA and protein levels of NF-kappaBp65 and MMP-2 were determined by reverse transcription-poly-merase chain reaction(RT-PCR) and by Western blot ,respectively .Results Compared with the SH group ,the values of the total collgen ,typeⅠcollgen ,typeⅢcollgen ,and Ⅰ /Ⅲ collgen ratio in the MI group and the PD group were significantly increased ,the differen had statistical significance (P<0 .01) .The values of the total collgen ,typeⅠcollgen ,typeⅢcollgen ,and Ⅰ /Ⅲ collgen ratio in the PD group were notably decreased than those in the MI group(P<0 .01) .Moreover ,the mRNA and protein levels of NF-kap-paBp65 and MMP-2 in the PD group were lower than those in the MI group ,the difference had statistical significance(P<0 .01) . Conclusion Left ventricular collagen remodeling following acute myocardial infarction could be improved by PDTC to some extent , which mechanism could be related with inhibiting NF-kappaB activation and down -regulating the expression of MMP-2 in rats .