CAJ | 학술논문

目的：研究LRP16在子宫内膜癌组织中的表达变化及其对子宫内膜癌 HEC-1-B细胞增殖的影响。方法：应用逆转录聚合酶链反应（RT-PCR）检测26例正常子宫内膜组织及10例子宫内膜癌组织中LRP16 mRNA的表达；LRP16转染细胞， RT-PCR证实转染的成功性，WST-1法观察细胞增殖的变化。结果子宫内膜癌组织中LRP16 mRNA的阳性表达率及表达水平（分别为83．33％、0．82±0．21）明显高于正常子宫内膜组织（分别为30．00％、0．47±0．18），二者比较差异有统计学意义（ P＜0．05）。RT-PCR检测结果显示转染后细胞LRP16 mRNA表达明显增加。转染组 HEC-1-B细胞在体外能继续增殖，但增殖能力并没有增强。结论： LRP16的异常表达可能与子宫内膜癌的发生、发展密切相关，LRP16基因用于子宫内膜癌基因治疗可能具有潜在价值。
목적：연구LRP16재자궁내막암조직중적표체변화급기대자궁내막암 HEC-1-B세포증식적영향。방법：응용역전록취합매련반응（RT-PCR）검측26례정상자궁내막조직급10례자궁내막암조직중LRP16 mRNA적표체；LRP16전염세포， RT-PCR증실전염적성공성，WST-1법관찰세포증식적변화。결과자궁내막암조직중LRP16 mRNA적양성표체솔급표체수평（분별위83．33％、0．82±0．21）명현고우정상자궁내막조직（분별위30．00％、0．47±0．18），이자비교차이유통계학의의（ P＜0．05）。RT-PCR검측결과현시전염후세포LRP16 mRNA표체명현증가。전염조 HEC-1-B세포재체외능계속증식，단증식능력병몰유증강。결론： LRP16적이상표체가능여자궁내막암적발생、발전밀절상관，LRP16기인용우자궁내막암기인치료가능구유잠재개치。
Objective To investigate the expression change of LRP16 in endometrial cancer tissues and its influence on the pro-liferation of human endometrial carcinoma HEC-1-B cells .Methods HEC-1-B cells were transfected with LRP16 .RT-PCR was used to examine the expression of LRP16 in 26 normal endometrium specimens ,10 endometrial cancer specimens .RT-PCR was used for verifying the transfection success .WES-T was used to observe the proliferation change of HEC-1-B cells .Results The positive expression rate and level of LRP16 mRNA in the endometrial cancer tissues were 83 .33% and 0 .82 ± 0 .21 ,which were significantly higher than 30 .00% ,0 .47 ± 0 .18 in the normal endometrium tissues(P<0 .05) .The RT-PCR detection results revealed that the expression of LRP16 mRNA after transfection was significantly increased .HEC-1-B cells in the transfection group could continued to proliferate in vitro ,but the proliferation capacity was not increased .Conclusion The expression abnormality of LRP16 may be closely related to the occurrence and progress of endometrial cancer ,LRP16 gene may have potential value for the endometrial canc-er gene therapy .