重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2013年
26期
3142-3144,3148
,共4页
谭微%陈小兰%王楚琼%姜泊%何继满
譚微%陳小蘭%王楚瓊%薑泊%何繼滿
담미%진소란%왕초경%강박%하계만
肝脏%重组人生长激素%STAT5%CIS%SOCS
肝髒%重組人生長激素%STAT5%CIS%SOCS
간장%중조인생장격소%STAT5%CIS%SOCS
liver%recombinant human growth hormone%STAT5%CIS%SOCS
目的研究慢性重组人生长激素(rhGH)刺激对幼鼠肝脏STAT5的影响及其分子机制。方法将32只幼鼠共分为4组:磷酸盐缓冲液(PBS)组、PBS+ single rhGH 组、chronic rhGH 组、chronic rhGH+ single rhGH 组。慢性生长激素刺激组chronic rhGH和chronic rhGH+single rhGH两组共16只小鼠每天按照1μg/g的剂量腹腔注射 rhGH ,对照组和 PBS+ single rhGH组16只小鼠每天腹腔注射等体积 PBS 0.1 mol/L ,共3周。注射完最后1次 rhGH和 PBS 16 h 后次日8:00,处死前30 min PBS组和chronic rhGH组小鼠注射PBS 0.1 mol/L ,PBS+single rhGH和chronic rhGH+single rhGH组小鼠按1μg/g注射单剂量rhGH。结果慢性生长激素刺激组小鼠(chronic rhGH和chronic rhGH+ single rhGH)与对照组小鼠(PBS组和 PBS+single rhGH)相比体质量明显增加;与 PBS组相比,chronic rhGH组小鼠肝脏GH信号通路内基础p-STAT5水平显著降低;凝胶迁移实验检测的chronic rhGH组小鼠肝脏内STAT5与DNA的结合能力显著低于PBS组;处死前30 min按1μg/g单剂量注射rhGH 的小鼠中,chronic rhGH+single rhGH组较 PBS+single rhGH组小鼠肝脏p-STAT5水平则明显降低;chronic rhGH组与对照组相比,CIS含量显著升高,而SOCS-2的含量则无明显差异。结论 rhGH 慢性刺激可抑制幼鼠肝脏细胞内STAT5的磷酸化,其机制可能与CIS含量的升高有关。
目的研究慢性重組人生長激素(rhGH)刺激對幼鼠肝髒STAT5的影響及其分子機製。方法將32隻幼鼠共分為4組:燐痠鹽緩遲液(PBS)組、PBS+ single rhGH 組、chronic rhGH 組、chronic rhGH+ single rhGH 組。慢性生長激素刺激組chronic rhGH和chronic rhGH+single rhGH兩組共16隻小鼠每天按照1μg/g的劑量腹腔註射 rhGH ,對照組和 PBS+ single rhGH組16隻小鼠每天腹腔註射等體積 PBS 0.1 mol/L ,共3週。註射完最後1次 rhGH和 PBS 16 h 後次日8:00,處死前30 min PBS組和chronic rhGH組小鼠註射PBS 0.1 mol/L ,PBS+single rhGH和chronic rhGH+single rhGH組小鼠按1μg/g註射單劑量rhGH。結果慢性生長激素刺激組小鼠(chronic rhGH和chronic rhGH+ single rhGH)與對照組小鼠(PBS組和 PBS+single rhGH)相比體質量明顯增加;與 PBS組相比,chronic rhGH組小鼠肝髒GH信號通路內基礎p-STAT5水平顯著降低;凝膠遷移實驗檢測的chronic rhGH組小鼠肝髒內STAT5與DNA的結閤能力顯著低于PBS組;處死前30 min按1μg/g單劑量註射rhGH 的小鼠中,chronic rhGH+single rhGH組較 PBS+single rhGH組小鼠肝髒p-STAT5水平則明顯降低;chronic rhGH組與對照組相比,CIS含量顯著升高,而SOCS-2的含量則無明顯差異。結論 rhGH 慢性刺激可抑製幼鼠肝髒細胞內STAT5的燐痠化,其機製可能與CIS含量的升高有關。
목적연구만성중조인생장격소(rhGH)자격대유서간장STAT5적영향급기분자궤제。방법장32지유서공분위4조:린산염완충액(PBS)조、PBS+ single rhGH 조、chronic rhGH 조、chronic rhGH+ single rhGH 조。만성생장격소자격조chronic rhGH화chronic rhGH+single rhGH량조공16지소서매천안조1μg/g적제량복강주사 rhGH ,대조조화 PBS+ single rhGH조16지소서매천복강주사등체적 PBS 0.1 mol/L ,공3주。주사완최후1차 rhGH화 PBS 16 h 후차일8:00,처사전30 min PBS조화chronic rhGH조소서주사PBS 0.1 mol/L ,PBS+single rhGH화chronic rhGH+single rhGH조소서안1μg/g주사단제량rhGH。결과만성생장격소자격조소서(chronic rhGH화chronic rhGH+ single rhGH)여대조조소서(PBS조화 PBS+single rhGH)상비체질량명현증가;여 PBS조상비,chronic rhGH조소서간장GH신호통로내기출p-STAT5수평현저강저;응효천이실험검측적chronic rhGH조소서간장내STAT5여DNA적결합능력현저저우PBS조;처사전30 min안1μg/g단제량주사rhGH 적소서중,chronic rhGH+single rhGH조교 PBS+single rhGH조소서간장p-STAT5수평칙명현강저;chronic rhGH조여대조조상비,CIS함량현저승고,이SOCS-2적함량칙무명현차이。결론 rhGH 만성자격가억제유서간장세포내STAT5적린산화,기궤제가능여CIS함량적승고유관。
Objective Toinvestigatetheeffectofchronicrecombinantgrowthhormone(rhGH)stimulationonhepaticSTAT5in healthy young mouse and its molecular mechanism .Methods 32 normal young mice were divided into 4 groups :PBS ,PBS+single rhGH ,chronic rhGH ,chronic rhGH+ single rhGH groups .The mice in the treatment groups(chronic rhGH and chronic rhGH +single rhGH )received intraperitoneal injection of rhGH at 1 μg/g body weight once daily for three weeks ;the mice in the control groups(PBS and PBS+single rhGH) were injected with sterile phosphate buffered saline(PBS ,0 .1 mol/L) .At next day 8:00 after 16 h of injection of last rhGH and PBS ,at 30 min before killing mouse ,PBS 0 .1 mol/L was injected in the PBS group and the chro-nic rhGH group ,while the single dose of rhGH 1 μg/g was injected in the PBS+single rhGH and the chronic rhGH+single rhGH group .Results Compared with the control mice(PBS group and PBS+single rhGH group) ,the body mass in the rhGH stimulation mice (chronic rhGH group and the chronic rhGH + single rhGH group) was significantly increased ;compared with the PBS group ,the base p-STAT5 level within the hepatic GH signal pathway in the chronic rhGH was significantly decreased ;the combi-ning capacity of hepatic STAT5 and DNA detected by EMSA in the chronic rhGH group was significantly lower than that in the PBS group ;in the mice injected by single dose of rhGH 1 μg/g at 30 min before killing mouse ,the hepatic p-STAT5 level in the chronic rhGH+single rhGH was significantly decreased compared with the chronic rhGH + single rhGH ;compared with the con-trol group ,the CIS content in the chronic rhGH was obviously increased ,while the SOCS-2 content had no obvious difference .Con-clusion Chronic rhGH stimulation can inhibit the phosphorylation of hepatic STAT 5 in normal young mouse ,which mechanism could be related with the increase of CIS content .