CAJ | 학술논문

目的观察胃癌组织中环氧化酶-2（COX-2）的表达情况，探讨cagA阳性幽门螺杆菌（Hp）感染与COX-2的关系。方法应用聚合酶链反应（PCR）检测42例胃癌患者胃癌组织、癌旁增生组织及癌旁正常胃黏膜组织中 Hp cagA的表达情况。结果胃癌组织COX-2蛋白阳性率明显高于癌旁增生组织及正常胃黏膜组织，差异有统计学意义（P＜0．01）。经 PCR基因扩增42例胃癌标本中 Hp cagA阳性25例，Hp cagA阴性17例。Hp cagA阳性组COX-2蛋白阳性表达率为88．0％（22/25），Hp ca-gA阴性组COX-2蛋白阳性表达率为35．3％（6/17），二者比较差异有统计学意义（P＜0．01）。结论胃癌组织中存在COX-2的异常表达，cagA阳性Hp感染可能通过调节COX-2的表达水平促进胃癌的发生、发展。
목적관찰위암조직중배양화매-2（COX-2）적표체정황，탐토cagA양성유문라간균（Hp）감염여COX-2적관계。방법응용취합매련반응（PCR）검측42례위암환자위암조직、암방증생조직급암방정상위점막조직중 Hp cagA적표체정황。결과위암조직COX-2단백양성솔명현고우암방증생조직급정상위점막조직，차이유통계학의의（P＜0．01）。경 PCR기인확증42례위암표본중 Hp cagA양성25례，Hp cagA음성17례。Hp cagA양성조COX-2단백양성표체솔위88．0％（22/25），Hp ca-gA음성조COX-2단백양성표체솔위35．3％（6/17），이자비교차이유통계학의의（P＜0．01）。결론위암조직중존재COX-2적이상표체，cagA양성Hp감염가능통과조절COX-2적표체수평촉진위암적발생、발전。
Objective To observe the cyclooxygenase-2(COX-2) expression of gastric tumor tissue ,and to study the relationship between cagA + Helicobacter pylori(Hp cagA+ ) infection and COX-2 .Methods 42 specimens of gastric cancer tissue (including gastric carcinoma ,paracancerous hyperplastic tissue and paracancerous normal gastric mucosa ) in our hospitals from January 2010 to August 2011 were collected and detected the Hp cagA expression by PCR .Results The COX-2 protein positive rate in the gas-tric tcancer tissue was significantly higher than that in the paracancerous hyperplastic tissue and normal gastric mucosa (P<0 .01) . Among 42 cases of gastric cancer specimens ,Hp cagA+ was in 25 cases ,Hp cagA - was in 17 cases .The COX-2 protein positive rate was 88 .0% (22/25) in the Hp cagA+ group and 35 .3% (6/17) in the Hp cagA - group .The COX-2 protein positive rate in the Hp cagA+ gastric tissue was significantly higher than that in the Hp cagA - gastric tissue(P<0 .01) .Conclusion Abnormal ex-pression of COX-2 exists in gastric tcancer tissue ,cagA+ Helicobacter pylori infection may promote the occurrence and development of gastric cancer by regulating the expression level of COX-2 .