CAJ | 학술논문

旨在得到一套快速提取椰子DNA的方法。采用CTAB小样法、SDS-CTAB法以及PVP法提取椰子组织的DNA，并通过后续的PCR扩增和酶切等实验比较了3种方法所提取的DNA质量的优劣。将CTAB小样法提取的DNA与SDS-CTAB法和PVP法提取的DNA进行了比较，CTAB小样法提取的DNA的OD260/OD280(1.839)较另外2种方法SDS-CTAB法(1.709)和PVP法(1.613)的值更高，CTAB小样法提取的DNA适合于PCR扩增，扩增的条带清晰，且特异性好。此外，CTAB小样法提取的DNA，酶切也较为均匀。CTAB小样法能高效地提取椰子的DNA，并且能一次提取大量的高质量DNA样品，因而能满足高通量的实验要求，同提取DNA能较好地满足下游的分子生物学研究。
지재득도일투쾌속제취야자DNA적방법。채용CTAB소양법、SDS-CTAB법이급PVP법제취야자조직적DNA，병통과후속적PCR확증화매절등실험비교료3충방법소제취적DNA질량적우렬。장CTAB소양법제취적DNA여SDS-CTAB법화PVP법제취적DNA진행료비교，CTAB소양법제취적DNA적OD260/OD280(1.839)교령외2충방법SDS-CTAB법(1.709)화PVP법(1.613)적치경고，CTAB소양법제취적DNA괄합우PCR확증，확증적조대청석，차특이성호。차외，CTAB소양법제취적DNA，매절야교위균균。CTAB소양법능고효지제취야자적DNA，병차능일차제취대량적고질량DNA양품，인이능만족고통량적실험요구，동제취DNA능교호지만족하유적분자생물학연구。
The study was carried out for developing a rapid method for DNA of extracting coconut. The quality of DNA extracted with mini-CTAB, SDS-CTAB and PVP method was compared. In this study, the comparison of three sets of coconut DNA, which were isolated separately by mini-CTAB and SDS-CTAB method or PVP method, showed that DNA isolated by mini-CTAB had higher OD260/OD280 (1.839) than that of SDS-CTAB (1.709) or PVP method (1.613). Meanwhile DNA isolated with the three methods was used for the downstream PCR amplification and restriction analysis. The DNA template from mini-CTAB method produced more clearer and specific bands. The double-enzyme cleavage of DNA from mini-CTAB method showed more even bands. The mini-CTAB method can be used to extract DNA from coconut tissues efficiently and multiple samples with high quality at a time. Since that this method could be used in high throughout experiments which including abundant samples. Furthermore, this method produced DNA which was suitable for downstream molecular biology experiment.