中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2013年
28期
14-16,17
,共4页
王春艳%王崇兰%王曙光%徐艳
王春豔%王崇蘭%王曙光%徐豔
왕춘염%왕숭란%왕서광%서염
减毒麻疹病毒%子宫内膜腺癌细胞%细胞凋亡%Fas%FasL%TGF-β
減毒痳疹病毒%子宮內膜腺癌細胞%細胞凋亡%Fas%FasL%TGF-β
감독마진병독%자궁내막선암세포%세포조망%Fas%FasL%TGF-β
Measle vaccinal virus%Apoptosis%Fas%FasL%TGF-β
目的：探讨体外感染减毒麻疹病毒（MV）对子宫内膜腺癌细胞（JEC）的凋亡诱导作用及相关机理。方法：MTT比色法动态监测MV感染对JEC细胞增殖活力的影响；琼脂糖凝胶电泳以及流式细胞术检测细胞凋亡；免疫细胞化学法检测MV感染后JEC细胞的Fas、FasL及TGF-β表达，分析凋亡机制。结果：MV感染6 d内，促进细胞的生长；感染6 d后，抑制细胞的生长，最终造成细胞死亡。流式细胞术结果显示：各感染浓度组JEC细胞的凋亡率比正常对照组增高（P<0.05或P<0.01），随感染时间的延长，细胞的凋亡率呈上升的趋势，感染12 d时，凋亡率与感染浓度呈正相关（r=0.77），且琼脂糖凝胶电泳出现特征性DNA“Ladder”凋亡条带；此时，感染细胞内Fas及TGF-β的表达增强（P<0.01），而FasL始终未表达。结论：MV感染能够抑制JEC细胞增值，并诱导其凋亡，JEC细胞凋亡机制可能与Fas、TGF-β的表达有关。
目的：探討體外感染減毒痳疹病毒（MV）對子宮內膜腺癌細胞（JEC）的凋亡誘導作用及相關機理。方法：MTT比色法動態鑑測MV感染對JEC細胞增殖活力的影響；瓊脂糖凝膠電泳以及流式細胞術檢測細胞凋亡；免疫細胞化學法檢測MV感染後JEC細胞的Fas、FasL及TGF-β錶達，分析凋亡機製。結果：MV感染6 d內，促進細胞的生長；感染6 d後，抑製細胞的生長，最終造成細胞死亡。流式細胞術結果顯示：各感染濃度組JEC細胞的凋亡率比正常對照組增高（P<0.05或P<0.01），隨感染時間的延長，細胞的凋亡率呈上升的趨勢，感染12 d時，凋亡率與感染濃度呈正相關（r=0.77），且瓊脂糖凝膠電泳齣現特徵性DNA“Ladder”凋亡條帶；此時，感染細胞內Fas及TGF-β的錶達增彊（P<0.01），而FasL始終未錶達。結論：MV感染能夠抑製JEC細胞增值，併誘導其凋亡，JEC細胞凋亡機製可能與Fas、TGF-β的錶達有關。
목적：탐토체외감염감독마진병독（MV）대자궁내막선암세포（JEC）적조망유도작용급상관궤리。방법：MTT비색법동태감측MV감염대JEC세포증식활력적영향；경지당응효전영이급류식세포술검측세포조망；면역세포화학법검측MV감염후JEC세포적Fas、FasL급TGF-β표체，분석조망궤제。결과：MV감염6 d내，촉진세포적생장；감염6 d후，억제세포적생장，최종조성세포사망。류식세포술결과현시：각감염농도조JEC세포적조망솔비정상대조조증고（P<0.05혹P<0.01），수감염시간적연장，세포적조망솔정상승적추세，감염12 d시，조망솔여감염농도정정상관（r=0.77），차경지당응효전영출현특정성DNA“Ladder”조망조대；차시，감염세포내Fas급TGF-β적표체증강（P<0.01），이FasL시종미표체。결론：MV감염능구억제JEC세포증치，병유도기조망，JEC세포조망궤제가능여Fas、TGF-β적표체유관。
Objective:To study the apoptosis-inducing effects and mechanism of infection with measle vaccinal virus on adenocarcinoma endometrium cell lines(JEC)in vitro.Method:The proliferation abilities of JEC cells affected by measle vaccinal virus was examined by MTT;the effects of apoptosis were detected using the following methods:DNA agarose gel electrophoresis,Annexin-V FITC/PI stained FCM;the expressions of Fas, FasL and TGF-βof JEC were examined by immunocytochemical staining.Result:(1)After infection,the results of MTT showed MV promoted the proliferation abilities of JEC cells in 6 days,then inhibited,at last induced the JEC cells to death after 6th day.And FITC/PI stained FCM showed that the percentage of the JEC apoptotic cells were higher than the negative control groups(P<0.05),and the apoptotic rate had the increasing tendency following with the prolonging of infected time;furthermore,at 12 day,the concentrations of virus were positive correlation with the apoptotic rate(r=0.77),at the same time,ladder-like pattern of DNA fragments were demonstrated by agarose gel electrophoresis. Simultaneously,The results of immunocytochemical staining method showed that the expression of Fas and TGF-βrose after infected with MV in JEC(P<0.01),but FasL had no expression always. Conclusion:These results indicate that measle vaccinal virus can inhibited the proliferation abilities and induce apoptosis in JEC cell line in vitro;the apoptotic mechanism may be associated with the expressions of Fas and TGF-β.
