重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2013年
27期
3234-3235,3238
,共3页
骨肉瘤%肝细胞生长因子%原癌基因蛋白质c-met%神经纤毛蛋白质1
骨肉瘤%肝細胞生長因子%原癌基因蛋白質c-met%神經纖毛蛋白質1
골육류%간세포생장인자%원암기인단백질c-met%신경섬모단백질1
osteosarcoma%hepatocyte grow th factor%proto-oncogene proteins c-met%neuropilin-1
目的检测骨肉瘤组织中肝细胞生长因子（HGF）、肝细胞生长因子受体（C-met）和神经纤毛蛋白1（NRP1）的表达特征，探讨三者的关系及临床意义。方法选择70例骨肉瘤术后留取的蜡块及临床资料作为观察组，选择50例成熟的骨组织脱钙后的蜡块及临床资料作为对照组，应免疫组织化学技术检测2组中HGF、C-met和NRP1蛋白的表达，探讨HGF、C-met和NRP1在肿瘤患者不同临床病理特征的表达差别及其相关性。结果观察组中HGF、C-met和NRP1蛋白表达的阳性率明显高于对照组，观察组中HGF、C-met和NRP1蛋白表达的阳性率与肿瘤的转移、脉管浸润、临床分期及Ki67的表达密切相关。观察组中HGF、C-met和NRP1之间的表达具有正相关性。结论骨肉瘤组织中HGF、C-met和NRP1高表达，三者对病变的进展具有重要作用，术后联合检测 HGF、C-met和NRP1的表达可能对判断预后有一定价值。
目的檢測骨肉瘤組織中肝細胞生長因子（HGF）、肝細胞生長因子受體（C-met）和神經纖毛蛋白1（NRP1）的錶達特徵，探討三者的關繫及臨床意義。方法選擇70例骨肉瘤術後留取的蠟塊及臨床資料作為觀察組，選擇50例成熟的骨組織脫鈣後的蠟塊及臨床資料作為對照組，應免疫組織化學技術檢測2組中HGF、C-met和NRP1蛋白的錶達，探討HGF、C-met和NRP1在腫瘤患者不同臨床病理特徵的錶達差彆及其相關性。結果觀察組中HGF、C-met和NRP1蛋白錶達的暘性率明顯高于對照組，觀察組中HGF、C-met和NRP1蛋白錶達的暘性率與腫瘤的轉移、脈管浸潤、臨床分期及Ki67的錶達密切相關。觀察組中HGF、C-met和NRP1之間的錶達具有正相關性。結論骨肉瘤組織中HGF、C-met和NRP1高錶達，三者對病變的進展具有重要作用，術後聯閤檢測 HGF、C-met和NRP1的錶達可能對判斷預後有一定價值。
목적검측골육류조직중간세포생장인자（HGF）、간세포생장인자수체（C-met）화신경섬모단백1（NRP1）적표체특정，탐토삼자적관계급림상의의。방법선택70례골육류술후류취적사괴급림상자료작위관찰조，선택50례성숙적골조직탈개후적사괴급림상자료작위대조조，응면역조직화학기술검측2조중HGF、C-met화NRP1단백적표체，탐토HGF、C-met화NRP1재종류환자불동림상병리특정적표체차별급기상관성。결과관찰조중HGF、C-met화NRP1단백표체적양성솔명현고우대조조，관찰조중HGF、C-met화NRP1단백표체적양성솔여종류적전이、맥관침윤、림상분기급Ki67적표체밀절상관。관찰조중HGF、C-met화NRP1지간적표체구유정상관성。결론골육류조직중HGF、C-met화NRP1고표체，삼자대병변적진전구유중요작용，술후연합검측 HGF、C-met화NRP1적표체가능대판단예후유일정개치。
Objective Todetect the expressions of HGF ,C-met and NRP1 protein in osteosarcoma and to investigate their corre-lation and the clinical significance .Methods The postoperative preserved paraffin block and the clinical data in 70 cases of osteosar-coma were taken as the observation group and 50 cases of the parraffin block of mature bone tissue after decalcification and the clin-ical data were taken as the control group .The expressions of HGF ,C-met and NRP1 protein were detected by the immunohisto-chemistry(IHC) method for investigating their expressions in the tumor patients with different clinicopathological characteristics and analyzing their relationship .Results The positive rates of HGF ,C-met and NRP1 expressions in the observation group were higher than those in the control group .The positive rates of HGF ,C-met and NRP1 expressions in the observation group were cor-related with metastasis ,vascular invasion ,clinical stage and Ki67 expression .The positive relationship was found between the ex-pressions of HGF and C-met with NRP1 of neoplasm .Conclusion The high expressions of HGF ,C-met and NRP1 in osteosarcoma play the important role in the progress of tumor lesion .The postoperative combined detection of HGF ,C-met and NRP1 protein could have certain value to predict the prognosis .
