CAJ | 학술논문

目的探讨PES1在卵巢癌中的表达及其与血管内皮生长因子（VEGF）表达的关系。方法用蛋白印迹法检测PES1在卵巢癌组织以及相应的癌旁组织中的表达。结果肿瘤组织中 PES1的表达水平明显高于相应的癌旁组织。转染FLAG-PES1的CAOV-3和 ES-2细胞培养液的 VEGF分泌量分别由（178．0±11．8） pg/mL和（309．5±18．5）pg/mL上升到（375．0±18．3）pg/mL和（633．2±25．7）pg/mL ，VEGF分泌量明显升高（P＜0．01）；而且VEGF 相对mRNA水平也分别上升了约1．8倍和2倍（P＜0．01）；蛋白印迹法结果表明，PES1过表达能升高这2种细胞中缺氧诱导因子-1α（HIF-1α）的表达。荧光素酶报告基因的转录激活活性检测表明，PES1对调节VEGF的转录没有直接影响。将HIF-1αSiRNA与FLAG-PES1共转染CA-OV-3和ES-2细胞后，蛋白印迹法结果表明，HIF-1αSiRNA能明显抑制PES1引起的 HIF-1α升高，同时VEGF的分泌量和mR-NA水平也被明显抑制。结论 PES1在卵巢癌组织中表达明显升高。
목적탐토PES1재란소암중적표체급기여혈관내피생장인자（VEGF）표체적관계。방법용단백인적법검측PES1재란소암조직이급상응적암방조직중적표체。결과종류조직중 PES1적표체수평명현고우상응적암방조직。전염FLAG-PES1적CAOV-3화 ES-2세포배양액적 VEGF분비량분별유（178．0±11．8） pg/mL화（309．5±18．5）pg/mL상승도（375．0±18．3）pg/mL화（633．2±25．7）pg/mL ，VEGF분비량명현승고（P＜0．01）；이차VEGF 상대mRNA수평야분별상승료약1．8배화2배（P＜0．01）；단백인적법결과표명，PES1과표체능승고저2충세포중결양유도인자-1α（HIF-1α）적표체。형광소매보고기인적전록격활활성검측표명，PES1대조절VEGF적전록몰유직접영향。장HIF-1αSiRNA여FLAG-PES1공전염CA-OV-3화ES-2세포후，단백인적법결과표명，HIF-1αSiRNA능명현억제PES1인기적 HIF-1α승고，동시VEGF적분비량화mR-NA수평야피명현억제。결론 PES1재란소암조직중표체명현승고。
Objective To investigate the expression of PES1 in ovarian cancer and its relationship with the vascular endothelial growth factor(VEGF) expression .Methods The expression of PES1 in ovarian cancer tissues and corresponding pericancerous tis-sues was detected with Western blot .Results The expression of PES1 in ovarian cancer tissues was obviously higher than that in the pericancerous tissues .The secretion amounts of VEGF in cell culture fluid of CAOV-3 and ES-2 with transfection of FLAG-PES1 were elevated from (178 .0 ± 11 .8) pg/mL and (309 .5 ± 18 .5) pg/mL to (375 .0 ± 18 .3) pg/mL and (633 .2 ± 25 .7)pg/mL respectively (P<0 .01) ,the secretion amounts of VEGF were also increased(P<0 .01) .The relative VEGF mRNA levels were also raised by 1 .8 times and 2 times respectively (P< 0 .01);Western blot simultaneously showed that the over expression of PES1 could increase the expression of HIF-1αin these two kinds of cells .The luciferase report gene transcriptional activation activity de-tection reveald that PES1 had no direct effect on the VEGF transcription .After cotransfecting HIF-1αSiRNA and FLAG-PES1 to CAOV-3 and ES-2 cells ,Western blot demonstrated that HIF-1αSiRNA could obviously inhibit the increase of HIF-1αexpression induced by PES1 ,at the same time the secretion amounts of VEGF and mRNA level were also supressed .Conclusion The expres-sion of PES1 is obviously up-regulated in ovarian cancer tissues .