中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2014年
6期
727-729
,共3页
朱宏伟%吴镜湘%陈旭%徐美英
硃宏偉%吳鏡湘%陳旭%徐美英
주굉위%오경상%진욱%서미영
硫化氢%肺移植%再灌注损伤%一氧化氮%一氧化氮合酶
硫化氫%肺移植%再灌註損傷%一氧化氮%一氧化氮閤酶
류화경%폐이식%재관주손상%일양화담%일양화담합매
Hydrogen sulfide%Lung transplantation%Reperfusion injury%Nitric oxide%Nitric oxide synthase
目的 评价外源性H2S对肺移植术大鼠肺缺血再灌注时一氧化氮(NO)/一氧化氮合酶的影响.方法 清洁级雄性SD大鼠40只,体重250 ～ 350 g,2～3月龄,采用随机数字表法,将其分为4组(n=10):假手术组(S组)、肺移植术组(L组)、肺移植术+ H2S生成酶胱硫醚-γ-裂解酶抑制剂炔丙基甘氨酸组(PPG组)和肺移植术+外源性H2S供体NaHS组(NaHS组).采用改良三袖套法制备大鼠左肺原位移植术模型,冷缺血时间为50 ～ 70 min;PPG组和NaHS组分别于移植肺肺门开放前5min腹腔注射炔丙基甘氨酸37.5 mg/kg和NaHS 14 μmol/kg.再灌注2h时取左肺组织,测定肺组织湿/干重(W/D)比值、丙二醛(MDA)、髓过氧化物酶(MPO)、诱导型一氧化氮合酶(iNOS)、内皮型一氧化氮合酶(eNOS)和NO的水平;光镜下观察肺组织病理学结果.结果 与S组比较,L组肺组织W/D比值、MDA、iNOS、NO和MPO的水平升高,肺组织eNOS含量降低(P＜0.05),NaHS组上述指标差异无统计学意义(P＞0.05);与L组比较,PPG组肺组织W/D比值、MDA、iNOS、NO和MPO的水平升高,肺组织eNOS含量降低,NaHS组肺组织W/D比值、MDA、iNOS、NO和MPO的水平降低,肺组织eNOS含量升高(P＜0.05).NaHS组肺组织病理学损伤明显轻于L组.结论 外源性H2S可减轻肺移植术大鼠肺缺血再灌注损伤,其机制与降低iNOS活性和升高eNOS活性有关.
目的 評價外源性H2S對肺移植術大鼠肺缺血再灌註時一氧化氮(NO)/一氧化氮閤酶的影響.方法 清潔級雄性SD大鼠40隻,體重250 ～ 350 g,2～3月齡,採用隨機數字錶法,將其分為4組(n=10):假手術組(S組)、肺移植術組(L組)、肺移植術+ H2S生成酶胱硫醚-γ-裂解酶抑製劑炔丙基甘氨痠組(PPG組)和肺移植術+外源性H2S供體NaHS組(NaHS組).採用改良三袖套法製備大鼠左肺原位移植術模型,冷缺血時間為50 ～ 70 min;PPG組和NaHS組分彆于移植肺肺門開放前5min腹腔註射炔丙基甘氨痠37.5 mg/kg和NaHS 14 μmol/kg.再灌註2h時取左肺組織,測定肺組織濕/榦重(W/D)比值、丙二醛(MDA)、髓過氧化物酶(MPO)、誘導型一氧化氮閤酶(iNOS)、內皮型一氧化氮閤酶(eNOS)和NO的水平;光鏡下觀察肺組織病理學結果.結果 與S組比較,L組肺組織W/D比值、MDA、iNOS、NO和MPO的水平升高,肺組織eNOS含量降低(P＜0.05),NaHS組上述指標差異無統計學意義(P＞0.05);與L組比較,PPG組肺組織W/D比值、MDA、iNOS、NO和MPO的水平升高,肺組織eNOS含量降低,NaHS組肺組織W/D比值、MDA、iNOS、NO和MPO的水平降低,肺組織eNOS含量升高(P＜0.05).NaHS組肺組織病理學損傷明顯輕于L組.結論 外源性H2S可減輕肺移植術大鼠肺缺血再灌註損傷,其機製與降低iNOS活性和升高eNOS活性有關.
목적 평개외원성H2S대폐이식술대서폐결혈재관주시일양화담(NO)/일양화담합매적영향.방법 청길급웅성SD대서40지,체중250 ～ 350 g,2～3월령,채용수궤수자표법,장기분위4조(n=10):가수술조(S조)、폐이식술조(L조)、폐이식술+ H2S생성매광류미-γ-렬해매억제제결병기감안산조(PPG조)화폐이식술+외원성H2S공체NaHS조(NaHS조).채용개량삼수투법제비대서좌폐원위이식술모형,랭결혈시간위50 ～ 70 min;PPG조화NaHS조분별우이식폐폐문개방전5min복강주사결병기감안산37.5 mg/kg화NaHS 14 μmol/kg.재관주2h시취좌폐조직,측정폐조직습/간중(W/D)비치、병이철(MDA)、수과양화물매(MPO)、유도형일양화담합매(iNOS)、내피형일양화담합매(eNOS)화NO적수평;광경하관찰폐조직병이학결과.결과 여S조비교,L조폐조직W/D비치、MDA、iNOS、NO화MPO적수평승고,폐조직eNOS함량강저(P＜0.05),NaHS조상술지표차이무통계학의의(P＞0.05);여L조비교,PPG조폐조직W/D비치、MDA、iNOS、NO화MPO적수평승고,폐조직eNOS함량강저,NaHS조폐조직W/D비치、MDA、iNOS、NO화MPO적수평강저,폐조직eNOS함량승고(P＜0.05).NaHS조폐조직병이학손상명현경우L조.결론 외원성H2S가감경폐이식술대서폐결혈재관주손상,기궤제여강저iNOS활성화승고eNOS활성유관.
Objective To evaluate the effects of exogenous hydrogen sulfide (H2S) on nitric oxide (NO) and nitric oxide synthase (NOS) during lung ischemia-reperfusion in a rat model of lung transplantation.Methods Forty pathogen-free male Sprague-Dawley rats,weighing 250-350 g,aged 2-3 months,were used as donor and recipient rats in this study.The animals were randomly divided into 4 groups (n =10 each):sham operation group (group S); lung transplantation group (group L); lung transplantation + H2S synthesis-CSE inhibitor D,L-propargylgylcine (PPG) group (group PPG); lung transplantation + exogenous H2S donor NaHS group (group NaHS).The donor lungs were removed from live donor rats and placed in low potassium dextran solution at 4 ℃.Orthotopic left lung transplantation was performed using modified three-cuff technique and the time for cold ischemia was 50-70 min.In PPG and NaHS groups,PPG 37.5 mg/kg and NaHS 14 μmol/kg were injected intraperitoneally at 5 min before opening of the hilum of the transplanted lung.The implanted donor lungs were ventilated and reperfused.The donor lungs were removed after being implanted,ventilated and reperfused for 2 h for microscopic examination and for determination of wet to dry lung weight (W/D) ratio and the levels of malondialdehyde (MDA),myeloperoxidase (MPO),inducible NOS (iNOS),endothelial NOS (eNOS),and NO.Results Compared with group S,W/D ratio and the levels of MDA,iNOS,NO and MPO were significantly increased,and the content of eNOS was decreased in group L,and no significant change was found in the parameters mentioned above in group NaHS.Compared with group L,W/D ratio and the levels of MDA,iNOS,NO and MPO were significantly increased,and the content of eNOS was decreased in group PPG,and W/D ratio and the levels of MDA,iNOS,NO and MPO were decreased,and the content of eNOS was increased in group NaHS.The pathological changes of lungs were significantly attenuated in group NaHS as compared with group L.Conclusion Exogenous H2S can attenuate the lung ischemia-reperfusion injury in a rat model of lung transplantation,and decreased iNOS activity and increased eNOS activity are involved in the mechanism.
