CAJ | 학술논문

目的研究卵泡刺激素(follicle stimulating hormone,FSH)体外对卵巢癌HEY细胞系Notch通路上Notch1胞内段蛋白(N1ICD)、转录因子CSL与靶基因表达以及对转录因子Snail家族的调节作用,以探讨FSH促进卵巢癌侵袭的可能机制.方法体外培养卵巢癌HEY细胞系,设PBS对照组和FSH 40mU/mL实验组,改良的Boyden小室检测两组细胞侵袭力,Western blot检测两组N1ICD、CSL、Snail、Slug蛋白表达,RT-PCR检测HEY-1、HEY-2、HES-1 mRNA表达.结果 FSH 40 mIU/mL可显著增强卵巢癌HEY细胞系的侵袭能力(P=0.001).相比于对照组,实验组FSH 40 mIU/mL作用24 h后N1ICD和CSL蛋白表达显著上升(P=0.001,P=0.028).FSH 40 mIU/mL作用后18～24 h HEY-1 mRNA的表达增加,作用24 h时HEY-2 mRNA的表达增加.而FSH作用后HES-1 mRNA水平无明显变化.FSH 40 mIU/mL作用24 h后,相比于对照组Snail蛋白表达显著上升(P=0.009),而Slug蛋白的表达无显著变化(P＞0.05).结论 FSH可能激活Notch1信号通路和Snail转录因子,进而促进卵巢癌细胞的侵袭能力.
목적 연구란포자격소(follicle stimulating hormone,FSH)체외대란소암HEY세포계Notch통로상Notch1포내단단백(N1ICD)、전록인자CSL여파기인표체이급대전록인자Snail가족적조절작용,이탐토FSH촉진란소암침습적가능궤제.방법 체외배양란소암HEY세포계,설PBS대조조화FSH 40mU/mL실험조,개량적Boyden소실검측량조세포침습력,Western blot검측량조N1ICD、CSL、Snail、Slug단백표체,RT-PCR검측HEY-1、HEY-2、HES-1 mRNA표체.결과 FSH 40 mIU/mL가현저증강란소암HEY세포계적침습능력(P=0.001).상비우대조조,실험조FSH 40 mIU/mL작용24 h후N1ICD화CSL단백표체현저상승(P=0.001,P=0.028).FSH 40 mIU/mL작용후18～24 h HEY-1 mRNA적표체증가,작용24 h시HEY-2 mRNA적표체증가.이FSH작용후HES-1 mRNA수평무명현변화.FSH 40 mIU/mL작용24 h후,상비우대조조Snail단백표체현저상승(P=0.009),이Slug단백적표체무현저변화(P＞0.05).결론 FSH가능격활Notch1신호통로화Snail전록인자,진이촉진란소암세포적침습능력.