CAJ | 학술논문

为了从分离得到的海洋真菌中筛选并鉴定具有杀松材线虫活性的菌株，本文采用稀释涂布平板法对采自青岛近海海域样品中的真菌进行分离，使用浸渍法对菌株的培养滤液和菌丝破碎液进行杀松材线虫活性测定，同时根据菌落形态、大小、色泽等特点的不同，经进一步纯化得到6个纯菌株，对这些菌株进行杀线活性测定，经初步筛选得到2株具有较强杀线活性的菌株，菌株HZ 11处理28 h时杀线活性最高，其培养滤液和菌丝破碎滤液的线虫校正死亡率分别达到72.6%和84.1%；菌株HZ 12处理32 h时杀线活性最高，其培养滤液和菌丝破碎滤液处理的线虫校正死亡率分别为46.3%和58.8%。选出活性表现最强的菌株HZ 11进行后续实验。实验结果表明，菌株HZ 11为轮枝孢属(Verticillium)的真菌。本研究对由松材线虫引起的松树萎蔫病的生物防治具有重要的理论意义。
위료종분리득도적해양진균중사선병감정구유살송재선충활성적균주，본문채용희석도포평판법대채자청도근해해역양품중적진균진행분리，사용침지법대균주적배양려액화균사파쇄액진행살송재선충활성측정，동시근거균락형태、대소、색택등특점적불동，경진일보순화득도6개순균주，대저사균주진행살선활성측정，경초보사선득도2주구유교강살선활성적균주，균주HZ 11처리28 h시살선활성최고，기배양려액화균사파쇄려액적선충교정사망솔분별체도72.6%화84.1%；균주HZ 12처리32 h시살선활성최고，기배양려액화균사파쇄려액처리적선충교정사망솔분별위46.3%화58.8%。선출활성표현최강적균주HZ 11진행후속실험。실험결과표명，균주HZ 11위륜지포속(Verticillium)적진균。본연구대유송재선충인기적송수위언병적생물방치구유중요적이론의의。
In order to screen and identify the fungal strains with nematicidal activity against the pine wood nematode,Barsaphelenchus xylophilus,from the fungi isolated from the marine environment,in this pa-per,the dilution and streak plate method was used to isolate fungal strains from the samples collected from the Yellow Sea near Qingdao.The culture filtrate and mycelial extract of the fungal strains isolated were assayed invitro for nematicidal activity using immersion test.At the same time,based on the coloni-al morphology,size,color and other characteristics,six pure strains were isolated.After preliminary screening,two strains with strong nematicidal activity were obtained.Strain HZ 1 1 showed the highest activity against B.xylophilus.The revised mortalities of B.xylophilus treated with the culture filtrate and mycelial extract of HZ 1 1 for 28h were 72.6% and 84.1%,respectively.Strain HZ 1 2 reached the highest nematicidal activity against B.xylophilus treated with the culture filtrate and mycelial extract for 32 h,and the corrected mortality were 46.3% and 58.8%,respectively.The strain HZ 1 1 with the highest activity was selected for further experiments.The experimental result showed that the fungal strain HZ 1 1 belonged to the genus of Verticillium.This study had important theoretical significance for the biological control of pine wilt disease caused by the pine wood nematode.