CAJ | 학술논문

采用胶原材料制备了一种密实-疏松双层结构的牙周引导再生膜材料．为改善再生膜材料的物理化学性能，采用1-乙基-3-（3-二甲基氨丙基）碳二亚胺（ EDC）和N-羟基琥珀酰亚胺（ NHS）为交联剂对膜材料进行交联，考察了不同交联剂质量浓度对膜材料物理化学性能的影响，并通过差示扫描量热分析仪（ DSC）、扫描电镜（ SEM）、吸水率测试、膨胀动力学分析、抗酶解性能分析等手段对膜材料交联前后的结构与性能进行了研究．结果表明，以EDC/NHS为交联剂，在pH为5.5、EDC质量浓度为5 g/L、交联时间为24 h的条件下，引导再生胶原膜材料的综合性能达到最佳，进一步提高交联剂的浓度，材料物理化学性能的变化并不明显．采用EDC/NHS交联后，可显著改善再生膜材料的物理化学性能．交联后膜材料的变性温度和抗酶解性能显著提高，并且维持了密实-疏松的双层结构．
채용효원재료제비료일충밀실-소송쌍층결구적아주인도재생막재료．위개선재생막재료적물이화학성능，채용1-을기-3-（3-이갑기안병기）탄이아알（ EDC）화N-간기호박선아알（ NHS）위교련제대막재료진행교련，고찰료불동교련제질량농도대막재료물이화학성능적영향，병통과차시소묘량열분석의（ DSC）、소묘전경（ SEM）、흡수솔측시、팽창동역학분석、항매해성능분석등수단대막재료교련전후적결구여성능진행료연구．결과표명，이EDC/NHS위교련제，재pH위5.5、EDC질량농도위5 g/L、교련시간위24 h적조건하，인도재생효원막재료적종합성능체도최가，진일보제고교련제적농도，재료물이화학성능적변화병불명현．채용EDC/NHS교련후，가현저개선재생막재료적물이화학성능．교련후막재료적변성온도화항매해성능현저제고，병차유지료밀실-소송적쌍층결구．
A collagen GTR membrane was made with a bi-layer structure , one of whose face is loose while the other is tight .The tight face can effectively prevent the cells from growing into the defective tis-sue zone while the loose face is rough , which is helpful to cells growth and blood clot .In order to im-prove the physicochemical properties of the membranes , EDC/NHS was used as a cross-linking agent for the membranes .And the effects of cross-linking agent dosage on the physicochemical properties of mem-branes were investigated .The measurements of DSC , SEM were used to test the structure and properties of the membranes .The properties of water absorption , collagen resistance were also tested .According to the study, 0.5 mol/L EDC/NHS, cross-linking 24 h in the pH of 5.5 are the best processing conditions . Increasing further the concentration of EDC can not improve the physicochemical properties of the mem-branes .The research shows that the physicochemical properties of collagen membranes are improved sig -nificantly after being cross-linked.The denaturation temperature , anti-collagen activities are both in-creased , and the bi-layer structure is kept well .