河南大学学报(医学版)
河南大學學報(醫學版)
하남대학학보(의학판)
JOURNAL OF HENAN UNIVERSITY (MEDICAL SCIENCE)
2013年
3期
190-192
,共3页
尿道外括约肌%运动终板%氯化金压片染色
尿道外括約肌%運動終闆%氯化金壓片染色
뇨도외괄약기%운동종판%록화금압편염색
urethral sphincter spinal%motor endplate%tablet of gold chloride staining
目的观察大鼠L6前根切断再吻合术后尿道外括约肌运动终板的重塑。方法选用SPF级成年雄性大鼠77只,随机分为3组,手术组L6前根切断后中枢端和外周端再吻合;假手术组L6前根切断后中枢端和外周端不吻合;正常组不做任何处理。将各组大鼠分别于术后1、4、8、16周取尿道外括约肌,应用氯化金压片染色的方法观察大鼠尿道外括约肌运动终板的形态学变化。结果术后1周手术组、假手术组尿道外括约肌运动终板形态与正常组无明显变化;术后4~8周手术组、假手术组尿道外括约肌运动终板形态均逐渐退变,但2组间无明显变化;术后16周手术组尿道外括约肌运动终板形态恢复接近正常组,假手术组则几乎看不到运动终板。结论 L6前根切断再吻合后,大鼠尿道外括约肌运动终板在术后16周前逐渐减少,16周后运动终板逐渐增多。
目的觀察大鼠L6前根切斷再吻閤術後尿道外括約肌運動終闆的重塑。方法選用SPF級成年雄性大鼠77隻,隨機分為3組,手術組L6前根切斷後中樞耑和外週耑再吻閤;假手術組L6前根切斷後中樞耑和外週耑不吻閤;正常組不做任何處理。將各組大鼠分彆于術後1、4、8、16週取尿道外括約肌,應用氯化金壓片染色的方法觀察大鼠尿道外括約肌運動終闆的形態學變化。結果術後1週手術組、假手術組尿道外括約肌運動終闆形態與正常組無明顯變化;術後4~8週手術組、假手術組尿道外括約肌運動終闆形態均逐漸退變,但2組間無明顯變化;術後16週手術組尿道外括約肌運動終闆形態恢複接近正常組,假手術組則幾乎看不到運動終闆。結論 L6前根切斷再吻閤後,大鼠尿道外括約肌運動終闆在術後16週前逐漸減少,16週後運動終闆逐漸增多。
목적관찰대서L6전근절단재문합술후뇨도외괄약기운동종판적중소。방법선용SPF급성년웅성대서77지,수궤분위3조,수술조L6전근절단후중추단화외주단재문합;가수술조L6전근절단후중추단화외주단불문합;정상조불주임하처리。장각조대서분별우술후1、4、8、16주취뇨도외괄약기,응용록화금압편염색적방법관찰대서뇨도외괄약기운동종판적형태학변화。결과술후1주수술조、가수술조뇨도외괄약기운동종판형태여정상조무명현변화;술후4~8주수술조、가수술조뇨도외괄약기운동종판형태균축점퇴변,단2조간무명현변화;술후16주수술조뇨도외괄약기운동종판형태회복접근정상조,가수술조칙궤호간불도운동종판。결론 L6전근절단재문합후,대서뇨도외괄약기운동종판재술후16주전축점감소,16주후운동종판축점증다。
Objective To observe the remodeling of motor endplate of the external urethral sphincter in rats after L 6 ventral root cut anastomosis . Methods 77 SPF adult male rats were randomly divided into three groups:surgery group:anastomosis of L6 ventral root centripetal end and peripheral end;sham group:L6 was cut off , but no anastomosis of L6 ventral root centripetal end and peripheral end was performed;normal group:no treatment . The external urethral sphincter was taken respectively in subgroups rats at 1 ,4 ,8 and 16 weeks after the operation . The gold chloride staining was used to observe the remodeling of motor endplate of the external urethral sphincter in rats .Results The morphology of urethral sphincter motor endplate in the 3 groups had no significant change after 1 week , that in surgery group and sham group gradually reduced after 4 W-8 W and there was no significant change of the morphology between the two groups . The morphology of urethral sphincter motor endplate in surgical group resumed nearly normal group after 16W postoperation , and the motor endplate could not be found in the sham group . Conclusion After cut and anastomosis of L6 ventral root , urethral sphincter motor endplate in rat gradually reduced before 16W postoperation , and gradually increased after 16W .