CAJ | 학술논문

建立了一种简便有效、成本低廉的去除子宫内膜癌血清高丰度蛋白的方法。运用3种不同的有机溶剂(不同体积倍数)处理子宫内膜癌血清样品， SDS-PAGE 电泳检测去除高丰度蛋白。结果表明：不同的有机溶剂沉淀法都能去除血清样品中绝大部分的高、中丰度蛋白，但低丰度蛋白质保留、减弱及丢失的现象不一。用1倍体积乙腈可有效去除子宫内膜癌血清样品中的绝大部分高丰度蛋白，同时保留较多的小分子量蛋白。
건립료일충간편유효、성본저렴적거제자궁내막암혈청고봉도단백적방법。운용3충불동적유궤용제(불동체적배수)처리자궁내막암혈청양품， SDS-PAGE 전영검측거제고봉도단백。결과표명：불동적유궤용제침정법도능거제혈청양품중절대부분적고、중봉도단백，단저봉도단백질보류、감약급주실적현상불일。용1배체적을정가유효거제자궁내막암혈청양품중적절대부분고봉도단백，동시보류교다적소분자량단백。
To establish a simple and effective method on eliminating high-abundance proteins in endometrial carcinoma serum. Elimination of serum high-abundance proteins were practiced by different organic solvents with different volume. The removal effects were analyzed by SDS-PAGE. Different organic solvent methods could eliminate most of high-abundance proteins in human serum, but the retention, weakening and loss of low-abundance protein is not the same. 1 times volume of acetonitrile could more effective eliminate high-abundance proteins, and gain more proteins low molecular weight.