CAJ | 학술논문

目的：观察维生素E对胃黏膜损伤的愈合干预。方法小鼠50只,随机分为5组,每组10只。正常对照组给予0.9%氯化钠溶液,其余组给予吲哚美辛20 mg·kg-1灌胃；4 h后,正常对照组和模型对照组给予0.9%氯化钠溶液,西咪替丁组给予西咪替丁200 mg·kg-1,维生素E低剂量组和高剂量组分别给予维生素E 25,50 mg·kg-1,每天灌胃一次。5 d后检测各组小鼠胃黏膜损伤程度及血清超氧化物歧化酶( SOD)活性、丙二醛( MDA)含量。结果西咪替丁组、维生素E低剂量组和高剂量组的胃黏膜损伤指数明显低于模型对照组,明显高于正常对照组(均P<0.01)；维生素E高剂量组的胃黏膜损伤指数明显低于西咪替丁组(P<0.05)；西咪替丁组、维生素E低剂量组和高剂量组的胃黏膜损伤愈合率明显高于模型对照组(均P<0.01)。维生素E低剂量组和高剂量组的血清SOD活性明显低于模型对照组和西咪替丁组,西咪替丁组的血清SOD活性明显高于正常对照组,低于模型对照组(均P<0.05)；西咪替丁组、维生素E低剂量组和高剂量组血清MDA含量均明显低于模型对照组(P<0.05或P<0.01)。结论维生素E可明显促进胃黏膜损伤愈合,其高剂量作用强于西咪替丁,低剂量作用与西咪替丁相当。
목적：관찰유생소E대위점막손상적유합간예。방법소서50지,수궤분위5조,매조10지。정상대조조급여0.9%록화납용액,기여조급여신타미신20 mg·kg-1관위；4 h후,정상대조조화모형대조조급여0.9%록화납용액,서미체정조급여서미체정200 mg·kg-1,유생소E저제량조화고제량조분별급여유생소E 25,50 mg·kg-1,매천관위일차。5 d후검측각조소서위점막손상정도급혈청초양화물기화매( SOD)활성、병이철( MDA)함량。결과서미체정조、유생소E저제량조화고제량조적위점막손상지수명현저우모형대조조,명현고우정상대조조(균P<0.01)；유생소E고제량조적위점막손상지수명현저우서미체정조(P<0.05)；서미체정조、유생소E저제량조화고제량조적위점막손상유합솔명현고우모형대조조(균P<0.01)。유생소E저제량조화고제량조적혈청SOD활성명현저우모형대조조화서미체정조,서미체정조적혈청SOD활성명현고우정상대조조,저우모형대조조(균P<0.05)；서미체정조、유생소E저제량조화고제량조혈청MDA함량균명현저우모형대조조(P<0.05혹P<0.01)。결론유생소E가명현촉진위점막손상유합,기고제량작용강우서미체정,저제량작용여서미체정상당。
Objective To investigate interventions of vitamin E( VE) on indomethacin-induced gastric mucosal injury. Methods Fifty mice were randomly divided into 5 groups. Normal control group was administrated with saline, others were intragastrically administrated with indomethacin ( 20 mg · kg-1 ) . After 4 hours, mice were intragastrically treated with saline, cimetidine(200 mg·kg-1),VE(25,50 mg·kg-1) once a day for 5 consecutive days. By the end of the treatment,gastric mucosa injury index,serum superoxide dismutase(SOD) activity,and malondialdehyde(MDA) content were determined. ResultsGastric mucosal injury indexs of mice treated with cimetidine,VE at low and high doses were significantly lower than that of the model control and higher than that of the normal control(P<0. 01). VE at high doses showed significantly less mucosal injury than that of the cimetidine group(P<0. 05). Healing-rate of gastric mucosal injury in cimetidine group,VE low-dose and high-dose groups were significantly higher than that of model control group(P<0. 01). SOD activity of VE at low and high doses was significantly lower than that of the model control and cimetidine groups(P<0. 05). The results also revealed that cimetidine and two doses of VE significantly elevated SOD activity and lowered the level of MDA(P<0. 05, 0. 01). Conclusion VE can obviously promote the healing of gastric mucosal injury,especially at high dose,which is better than cimetidine,while,the low dose is comparable with cimetidine.