CAJ | 학술논문

采用实时荧光聚合酶链反应（PCR ）检测性病患者泌尿生殖道沙眼衣原体，进行临床和实验室分析。采集380例男女性病患者泌尿生殖道分泌物，进行多形核白细胞数检测和荧光PCR。结果显示，216例男性患者中，尿道多形核白细胞数≥5个者占92．59％，荧光PCR检测沙眼衣原体阳性62例，阳性率为28．70％；沙眼衣原体合并淋病奈瑟菌感染30例，合并感染率为13．89％。164例女性患者中，87例宫颈管内多形核白细胞数≥10个（占53．05％），荧光PCR检测沙眼衣原体阳性33例（占20．12％）。结果提示，性病门诊开展实时荧光PCR检测沙眼衣原体可提高检测阳性率和控制沙眼衣原体传播。
채용실시형광취합매련반응（PCR ）검측성병환자비뇨생식도사안의원체，진행림상화실험실분석。채집380례남녀성병환자비뇨생식도분비물，진행다형핵백세포수검측화형광PCR。결과현시，216례남성환자중，뇨도다형핵백세포수≥5개자점92．59％，형광PCR검측사안의원체양성62례，양성솔위28．70％；사안의원체합병임병내슬균감염30례，합병감염솔위13．89％。164례녀성환자중，87례궁경관내다형핵백세포수≥10개（점53．05％），형광PCR검측사안의원체양성33례（점20．12％）。결과제시，성병문진개전실시형광PCR검측사안의원체가제고검측양성솔화공제사안의원체전파。
380 urogenital tract secretion samples from patients with sexually transmitted diseases were subjected for Chlamydia trachomatis detection by polymorphonuclear leukocyte count and real-time fluorescent polymerase chain reaction (PCR ) . The results showed that of 216 male patients 92 .59%presented urethral polymorphonuclear leukocyte count ≥ 5 , and 62 cases (28 .70% ) were Chlamydia trachomatis-positive detected by PCR . There were 30 cases with Chlamydia trachomatis and Neisseria gonorrhoeae coinfection (13 .89% ) . Of 164 female patients , 53 .05% presented cervical canal polymorphonuclear leukocyte count ≥ 10 , and 33 cases (20 .12% ) were Chlamydia trachomatis-positive detected by PCR .The results indicated that the application of real-time fluorescent PCR based assay can improve the clinical management of Chlamydia trachomatis .