CAJ | 학술논문

目的研究新佐剂-Montanide ISA 50V2在马免疫中的应用，制定合理的免疫程序，提高马免疫血清效价。方法用Montanide ISA 50V2乳化A型和F型肉毒类毒素制备成油乳抗原，免疫家兔和马，并与福氏不完全佐剂进行对比。结果在马免疫程序中，Montanide ISA 50V2与福氏不完全佐剂交替使用效果更好，免疫效价较高。结论在进一步试验确证后，将Montanide ISA 50V2作为佐剂应用于马免疫中，以获得更高的免疫血清的效价。
목적연구신좌제-Montanide ISA 50V2재마면역중적응용，제정합리적면역정서，제고마면역혈청효개。방법용Montanide ISA 50V2유화A형화F형육독류독소제비성유유항원，면역가토화마，병여복씨불완전좌제진행대비。결과재마면역정서중，Montanide ISA 50V2여복씨불완전좌제교체사용효과경호，면역효개교고。결론재진일보시험학증후，장Montanide ISA 50V2작위좌제응용우마면역중，이획득경고적면역혈청적효개。
Objective To improve the titers of antitoxin serum prepared in horses immunization program .Methods Mon-tanide ISA 50V2 was used as a new adjuvant in immunization program for rabbits and horses .Montanide SA 50V2 and Clostridium botulinum type A and F toxoids antigens were emulsified to O /W emulsion, and then using this emulsion to im-munize the horses , and compared with the conventional adjuvant ( FIA) with the same antigen of clostridium botulinum type A and F toxoids .Results The immune effect was the best when two emulsions were alternately used ,the titer of antitoxin serum was the highest .Conclusion Montanide ISA 50V2 could be used as a adjuvant in horses immunization program for production of higher titers of antitoxin serum .