CAJ | 학술논문

目的建立肺炎链球菌发酵过程中的质控方法。方法应用革兰染色镜检与荚膜肿胀试验监测菌种的退化及发酵罐染菌情况，或用于退化菌种的复壮；应用发酵过程中测定培养液光密度（ A600）的方法监测发酵状况、确定培养液初始浓度以及收获时间；应用血糖仪测定发酵过程中的葡萄糖含量，保证发酵过程中碳源的及时补充；应用渗透压仪检测发酵过程中培养液渗透压的变化，确定补料策略。结果确立了细菌革兰染色、荚膜肿胀试验、葡萄糖含量、渗透压、光密度（ A600）等作为肺炎链球菌发酵过程中的质控方法。结论实验研究中建立的肺炎链球菌发酵过程质控方法能够较好地监测和评价肺炎链球菌的发酵过程。
목적건립폐염련구균발효과정중적질공방법。방법응용혁란염색경검여협막종창시험감측균충적퇴화급발효관염균정황，혹용우퇴화균충적복장；응용발효과정중측정배양액광밀도（ A600）적방법감측발효상황、학정배양액초시농도이급수획시간；응용혈당의측정발효과정중적포도당함량，보증발효과정중탄원적급시보충；응용삼투압의검측발효과정중배양액삼투압적변화，학정보료책략。결과학립료세균혁란염색、협막종창시험、포도당함량、삼투압、광밀도（ A600）등작위폐염련구균발효과정중적질공방법。결론실험연구중건립적폐염련구균발효과정질공방법능구교호지감측화평개폐염련구균적발효과정。
Objective To develop a set of parameters for quality control of Streptococcus pneumoniae in the fermentation process .Methods Gram staining and capsule swelling test were used for monitoring bacterial degeneration and contamina -tion by other microorganisms in the fermentor , or for rejuvenation of degenerated seed strains .The optical density ( A600 ) in fermentation broth was determinated for monitoring the fermentation process and confirming the initial concentration of fer -mentation broth and the harvest time .The content of glucose was detected by a glucose meter at intervals so as to make sure supplementary carbon source was timely supplied in fermentation process .Osmotic pressure was measured by an osmometer in fermentation process in order to guide the strategy of feeding supplement .Results The results from Gram stain , capsule swelling test, the content of glucose, osmolality and optical density (A600) were determined as parameters in quality control during the the fermentation of Streptococcus pneumoniae.Conclusion The fermentation process of Streptococcus pneumoni-ae can be evaluated and monitored by a developed quality control method in this study .