CAJ | 학술논문

对5种双壳类软体动物(近江蛏Sinonovacula rivularis、缢蛏Sinonovacula constricta、小刀蛏Cultellus attenuatus、尖刀蛏Cultellus scalprum和大竹蛏Solen grandis)的线粒体基因COⅠ和16S rRNA部分序列进行测序和分析,并结合GenBank中其他竹蛏超科和樱蛤超科物种COⅠ和16S rRNA片段,计算种间遗传距离,构建系统发育树,探讨近江蛏及缢蛏属的分类地位。结果表明,5个物种COⅠ和16S rRNA片段A+T含量均远高于 G+C 含量,近江蛏与缢蛏之间的碱基序列差异和遗传距离均已达到种间差异水平,确定近江蛏为缢蛏属的一个种。分别构建COⅠ(砂海螂为外群)和16S rRNA片段(密鳞牡蛎为外群)的Neighbor-Joining系统树,两者的拓扑结构都明确显示,缢蛏属为灯塔蛤科一个属,灯塔蛤科录属于竹蛏超科,而不录属于樱蛤超科。
대5충쌍각류연체동물(근강정Sinonovacula rivularis、액정Sinonovacula constricta、소도정Cultellus attenuatus、첨도정Cultellus scalprum화대죽정Solen grandis)적선립체기인COⅠ화16S rRNA부분서렬진행측서화분석,병결합GenBank중기타죽정초과화앵합초과물충COⅠ화16S rRNA편단,계산충간유전거리,구건계통발육수,탐토근강정급액정속적분류지위。결과표명,5개물충COⅠ화16S rRNA편단A+T함량균원고우 G+C 함량,근강정여액정지간적감기서렬차이화유전거리균이체도충간차이수평,학정근강정위액정속적일개충。분별구건COⅠ(사해랑위외군)화16S rRNA편단(밀린모려위외군)적Neighbor-Joining계통수,량자적탁복결구도명학현시,액정속위등탑합과일개속,등탑합과록속우죽정초과,이불록속우앵합초과。
Partial sequences of mitochondrial gene COⅠ and 16S rRNA from five bivalve species (Sinonovacula rivu-laris, Sinonovacula constricta, Cultellus attenuatus, Cultellus scalprum and Solen grandis) were sequenced and ana-lyzed. In the context of the sequences of other species of Solenoidae and Tellinoidea from GenBank, genetic distances were calculated. Phylogenetic trees (Neighbor-Joining) were constructed to determine the taxonomic position of Si-nonovacula rivularis and genus Sinonovacula. The results showed that the A+T content in COⅠ or 16S rRNA frag-ments was far more than that of G+C. Sequence difference and genetic distance between S. rivularis and S. constricta in COⅠ and 16S rRNA reached a level of interspecies difference, and thus S. rivularis was confirmed to be a separated species of genus Sinonovacula. The topological structures of phylogenetic trees constructed on the basis of COⅠ and 16S rRNA fragments with Mya arenaria or Ostrea denselamellosa as the outgroup revealed that Sinonovacula, which was a genus of Pharella, should be incorporated into Solenoidae, but not Tellinoidea.