内蒙古民族大学学报（自然科学版）
內矇古民族大學學報（自然科學版）
내몽고민족대학학보（자연과학판）
JOURNAL OF INNER MONGOLIA UNIVERSITY FOR NATIONALITIES(NATURAL SCIENCES)
2013年
4期
420-423
,共4页
蓖麻%PsbO基因%克隆%序列分析
蓖痳%PsbO基因%剋隆%序列分析
비마%PsbO기인%극륭%서렬분석
Castor%PsbO genes%Cloning%Sequence Analysis
用实验室栽培的蓖麻新鲜叶片为材料，Trizol法提取总RNA，参照GenBank公布的植物PsbO氨基酸序列，进行序列比对找出氨基酸序列保守区设计简并引物，通过RT-PCR扩增目的片段，纯化后克隆到T-Vector中测序，获得蓖麻的PsbO基因，对其基因序列进行分析。了解到其长度为1081bp，5＇非编码区为10bp，3＇非编码区为273bp，编码区长798bp，编码266个氨基酸。序列分析结果表明，蓖麻PsbO基因编码的氨基酸序列与已公布的其他植物中克隆到的PsbO氨基酸序列有较高的同源性。
用實驗室栽培的蓖痳新鮮葉片為材料，Trizol法提取總RNA，參照GenBank公佈的植物PsbO氨基痠序列，進行序列比對找齣氨基痠序列保守區設計簡併引物，通過RT-PCR擴增目的片段，純化後剋隆到T-Vector中測序，穫得蓖痳的PsbO基因，對其基因序列進行分析。瞭解到其長度為1081bp，5＇非編碼區為10bp，3＇非編碼區為273bp，編碼區長798bp，編碼266箇氨基痠。序列分析結果錶明，蓖痳PsbO基因編碼的氨基痠序列與已公佈的其他植物中剋隆到的PsbO氨基痠序列有較高的同源性。
용실험실재배적비마신선협편위재료，Trizol법제취총RNA，삼조GenBank공포적식물PsbO안기산서렬，진행서렬비대조출안기산서렬보수구설계간병인물，통과RT-PCR확증목적편단，순화후극륭도T-Vector중측서，획득비마적PsbO기인，대기기인서렬진행분석。료해도기장도위1081bp，5＇비편마구위10bp，3＇비편마구위273bp，편마구장798bp，편마266개안기산。서렬분석결과표명，비마PsbO기인편마적안기산서렬여이공포적기타식물중극륭도적PsbO안기산서렬유교고적동원성。
In the study,leaves of castor cultivated in the laboratory were used as materials then extracted the total RNA by Trizol method. The primer was designed through the conservative amino acid sequences of known plant PsbO. Pur-pose fragment was amplified with RT-PCR ,then cloned into T-Vector and sequenced.The results as fo-llows:the length of cloned PsbO was 1081bp,5 'noncoding region was10 bp,3' noncoding region was 273 bp and coding region was 798 bp,it could encode 266 amino acids. The results of sequence comparison indicated that the amino acids se-quence of PsbO from castor shares high sequence homology with the selected PsbOamino acids of other plants.
