化学分析计量
化學分析計量
화학분석계량
CHEMICAL ANALYSIS AND METERAGE
2013年
4期
63-65
,共3页
巩志国%苏敏%王静静%员丽娟%李世雨%季新成%朱建民
鞏誌國%囌敏%王靜靜%員麗娟%李世雨%季新成%硃建民
공지국%소민%왕정정%원려연%리세우%계신성%주건민
五灵脂%原儿茶酸%加速溶剂萃取仪(ASE)%液相色谱
五靈脂%原兒茶痠%加速溶劑萃取儀(ASE)%液相色譜
오령지%원인다산%가속용제췌취의(ASE)%액상색보
faeces trogopterori%protocatechuic acid%accelerated solvent extraction%liquid chromatography
建立了五灵脂中原儿茶酸含量的测定方法。利用加速溶剂萃取仪(ASE),以75%乙醇水溶液提取样品中的待测物,用Diamosil C18(4.6 mm×250 mm,5.0μm)色谱柱在梯度洗脱条件下分离待测物,外标法定量。原儿茶酸的线性范围为0.1~10.0 mg/L,相关系数r=0.9998。方法的回收率为83%~107%,测定结果的相对标准偏差为4.6%~10.5%,方法的定量限为1.0 mg/kg。该方法操作简便、快速,提取效率高,适用于检测和分析五灵脂中原儿茶酸的含量。
建立瞭五靈脂中原兒茶痠含量的測定方法。利用加速溶劑萃取儀(ASE),以75%乙醇水溶液提取樣品中的待測物,用Diamosil C18(4.6 mm×250 mm,5.0μm)色譜柱在梯度洗脫條件下分離待測物,外標法定量。原兒茶痠的線性範圍為0.1~10.0 mg/L,相關繫數r=0.9998。方法的迴收率為83%~107%,測定結果的相對標準偏差為4.6%~10.5%,方法的定量限為1.0 mg/kg。該方法操作簡便、快速,提取效率高,適用于檢測和分析五靈脂中原兒茶痠的含量。
건립료오령지중원인다산함량적측정방법。이용가속용제췌취의(ASE),이75%을순수용액제취양품중적대측물,용Diamosil C18(4.6 mm×250 mm,5.0μm)색보주재제도세탈조건하분리대측물,외표법정량。원인다산적선성범위위0.1~10.0 mg/L,상관계수r=0.9998。방법적회수솔위83%~107%,측정결과적상대표준편차위4.6%~10.5%,방법적정량한위1.0 mg/kg。해방법조작간편、쾌속,제취효솔고,괄용우검측화분석오령지중원인다산적함량。
The method was developed for the purification method of determination of protocatechuic acid in faeces trogopterori. The analyst was extracted from the samples with menthol-water using accelerated solvent extraction. The C18 column(4.6 mm×250 mm,5.0μm)was used to complete the separation of the analyst under gradient elution. The ex-ternal standard calibration curves were used for the quantitative determination. The linear range was from 0.1 to 10.0 mg/L with a good linear relationship(r=0.999 8). The limits of quantification(LOQ)was 1.0 mg/kg for protocatechuic acid. The recovery ranged from 83%to 107%with the relative standard deviations(RSD)between 4.6%and 10.5%. It was indicated that this method was simple,convenient,faster, and it had high efficient extraction. The method is suitable for determining protocatechuic acid in faeces trogopterori.