CAJ | 학술논문

采用毛细管气相色谱法测定鱼油中DHA和EPA的含量，色谱柱为DB-WAX型毛细管柱（30 m×0.530 mm，1.00μm），柱温采用程序升温，载气为氮气，流速15 mL/min，FID检测器，温度为300℃，进样口温度260℃，分流比为10∶1，进样量为1μL。DHA及EPA的质量浓度(X)分别在0.25～20.0，0.125～10.0 mg/mL范围内与色谱峰面积(Y)呈良好的线性关系，线性方程分别为Y=905417X+6060.6，Y=887335X+4463.6,线性相关系数r均为0.9999，平均回收率分别为97.8%，96.1%，相对标准偏差小于3％(n=6)。该方法简便、重现性好，可用于鱼油中DHA和EPA含量测定。
채용모세관기상색보법측정어유중DHA화EPA적함량，색보주위DB-WAX형모세관주（30 m×0.530 mm，1.00μm），주온채용정서승온，재기위담기，류속15 mL/min，FID검측기，온도위300℃，진양구온도260℃，분류비위10∶1，진양량위1μL。DHA급EPA적질량농도(X)분별재0.25～20.0，0.125～10.0 mg/mL범위내여색보봉면적(Y)정량호적선성관계，선성방정분별위Y=905417X+6060.6，Y=887335X+4463.6,선성상관계수r균위0.9999，평균회수솔분별위97.8%，96.1%，상대표준편차소우3％(n=6)。해방법간편、중현성호，가용우어유중DHA화EPA함량측정。
The content of DHA and EPA in fish oil was determined by capillary gas chromatographic method. The chromatographic column was DB-WAX(30 m×0.530 mm, 1.00 μm).The column temperature was programmed .The carrier gas was nitrogen with the flow rate at 15 mL/min.The detector was FID and its temperature was 300℃.The injection temperature was 260℃,the split ratio was 10∶1,and the injection volume was 1μL. The mass concentration of DHA methylester and EPA methylester was linear with peak area in the range of 0.25-20.0 mg/mL and 0.125-10.0 mg/mL respectively,the linear equation were Y=905 417X+6 060.6, Y=887 335X+4 463.6,and regression coefficient was 0.999 9. The average recovery were 97.8%, 96.1%,respectively, and the relative standard deviation was not more than 3%(n=6). The method is simple and convenient, it can be used to determine the content of DHA and EPA fish oils with good accuracy and repeatability.