CAJ | 학술논문

建立液相色谱-串联质谱(LC-MS/MS)法检测动物性食品中万古霉素药物残留的方法。用磷酸盐缓冲溶液对动物性食品中的万古霉素进行提取，经HLB固相萃取柱净化，采用电喷雾离子源，以正离子检测方式进行质谱分析。实验结果表明，万古霉素质量浓度在1～500μg/L范围内与色谱峰面积呈良好的线性，相关系数r＝0.998。低、中、高3个质量浓度添加水平的回收率为84.8%～118.2%，相对标准偏差为2.2%～7.2%（n=5），检出限为2μg/kg。
건립액상색보-천련질보(LC-MS/MS)법검측동물성식품중만고매소약물잔류적방법。용린산염완충용액대동물성식품중적만고매소진행제취，경HLB고상췌취주정화，채용전분무리자원，이정리자검측방식진행질보분석。실험결과표명，만고매소질량농도재1～500μg/L범위내여색보봉면적정량호적선성，상관계수r＝0.998。저、중、고3개질량농도첨가수평적회수솔위84.8%～118.2%，상대표준편차위2.2%～7.2%（n=5），검출한위2μg/kg。
The method for determining vancomycin in animal foods by LC-MS/MS was established. Vancomycin was extracted from animal foods by phosphate buffer, then purified by HLB solid phase extraction cartridges. Electro-spray ionization was used for mass spectrographic analysis by using positive ion. The experimental results showed that vancomycin mass concentration was linear with the chromatograph peak area in the range of 1-500μg/L with the correlation coefficient of 0.998. Vancomycin standard was added in low,medium and high concentration,the average recovery was 84.8%-118.2%and relative standard deviations ranged from 2.2%to 7.2%. The detection limit was 2μg/kg.