CAJ | 학술논문

为研究湖泊沉积物不同深度处的细菌群落结构组成和多样性，选取太湖梅梁湾作为采样点，应用末端限制性片段长度多态性（ T-RFLP）技术比较湖泊不同深度沉积物样品中细菌群落的结构组成及其多样性。结果表明：太湖沉积物中细菌具有丰富的多样性，细菌种类和相对丰度与当前已有的可培养细菌研究结果一致。细菌群落结构随着沉积物深度的增加呈现一定的变化规律，相邻近的沉积物分层中细菌群落结构的相似度较高；随着沉积物深度的增加，沉积物中优势菌属发生了一定的变化；表层沉积物与底层沉积物的细菌群落结构相差较大。鉴于氧含量随着沉积物深度的增加而逐渐减少，可以推断氧含量的变化是造成湖泊沉积物中细菌群落结构与多样性差异的主要原因。另外，表层沉积物中的生物扰动也是可能的原因。
위연구호박침적물불동심도처적세균군락결구조성화다양성，선취태호매량만작위채양점，응용말단한제성편단장도다태성（ T-RFLP）기술비교호박불동심도침적물양품중세균군락적결구조성급기다양성。결과표명：태호침적물중세균구유봉부적다양성，세균충류화상대봉도여당전이유적가배양세균연구결과일치。세균군락결구수착침적물심도적증가정현일정적변화규률，상린근적침적물분층중세균군락결구적상사도교고；수착침적물심도적증가，침적물중우세균속발생료일정적변화；표층침적물여저층침적물적세균군락결구상차교대。감우양함량수착침적물심도적증가이축점감소，가이추단양함량적변화시조성호박침적물중세균군락결구여다양성차이적주요원인。령외，표층침적물중적생물우동야시가능적원인。
To investigate the vertical distribution and diversity of bacterial community composition in the sediments of Taihu Lake , sediment samples were collected from Meiliang Bay in Taihu Lake .Terminal restriction fragment length polymorphism ( T-RFLP) technology was used to analyze the bacterial community composition and diversity in sediment samples of different depths .High bacterial diversities were observed in the sediments of Taihu Lake , and the bacterial species and relative abundance were found to be consistent with those in previous reports based on the culture-dependent method .Additionally , different T-RFLP patterns were observed for samples collected at different depths .Bacterial community compositions derived from those nearby samples showed greater similarities . The dominant bacterial groups varied at different depths .Moreover, the bacterial community compositions of the top and the bottom sediments showed great differences .Dissolved oxygen decreased with the increase of the depth of sediments, which shows that the variation of dissolved oxygen was the main cause of the differences in bacterial community compositions and diversity .Bioturbation induced by the submerged macrophytes and zoobenthos may be another cause .