CAJ | 학술논문

目的：探讨和厚朴酚（HNK）对四氯化碳（CCl4）诱导的肝损伤的保护作用。方法：将50只小鼠随机分成HNK高、中、低剂量组（分别以20．0、2．0、0．2mg/kg剂量皮下注射HNK注射液），正常组和模型组（注射等量生理盐水）5组。给药7d后，模型组和HNK各剂量组腹腔注射含CCl4的大豆油溶液，正常组注射等体积大豆油溶液。24h后，摘除眼球取血，解剖肝脏，观察小鼠肝脏的病理学变化，应用生化分析仪测定血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、血糖（Glu）含量，Real-timePCR法分析血清中ALT、AST、ALP和肝脏中肿瘤坏死因子-α（TNF-α）、白介素-6（IL-6）、干扰素-γ（IFN-γ）mRNA的表达。结果：HE染色结果显示，正常小鼠肝组织结构完整，肝细胞呈索状结构排列，肝细胞分界清晰，未见脂肪变性和炎性细胞浸润。模型组小鼠肝组织肝索排列紊乱，中央静脉变形，肝细胞肿胀并呈泡状脂肪变性，伴淋巴细胞和中性粒细胞浸润。HNK各剂量组上述病理变化均有不同程度减轻，尤以HNK高剂量组改善较明显。与模型组相比，HNK处理后，小鼠血清中ALT、AST、ALP含量和mRNA水平及Glu浓度均明显降低（F分别为274．620，1121．720；355．870，69．430；294．280，35．330；75．770；P均＜0．001）；同时，肝脏组织中TNF-α、IL-6和IFN-γmRNA的表达明显降低（F分别为1165．950，69．460，4787．190；P均＜0．001）。结论：HNK对CCl4诱导的小鼠急性肝损伤有保护作用。
목적：탐토화후박분（HNK）대사록화탄（CCl4）유도적간손상적보호작용。방법：장50지소서수궤분성HNK고、중、저제량조（분별이20．0、2．0、0．2mg/kg제량피하주사HNK주사액），정상조화모형조（주사등량생리염수）5조。급약7d후，모형조화HNK각제량조복강주사함CCl4적대두유용액，정상조주사등체적대두유용액。24h후，적제안구취혈，해부간장，관찰소서간장적병이학변화，응용생화분석의측정혈청중곡병전안매（ALT）、곡초전안매（AST）、감성린산매（ALP）、혈당（Glu）함량，Real-timePCR법분석혈청중ALT、AST、ALP화간장중종류배사인자-α（TNF-α）、백개소-6（IL-6）、간우소-γ（IFN-γ）mRNA적표체。결과：HE염색결과현시，정상소서간조직결구완정，간세포정색상결구배렬，간세포분계청석，미견지방변성화염성세포침윤。모형조소서간조직간색배렬문란，중앙정맥변형，간세포종창병정포상지방변성，반림파세포화중성립세포침윤。HNK각제량조상술병리변화균유불동정도감경，우이HNK고제량조개선교명현。여모형조상비，HNK처리후，소서혈청중ALT、AST、ALP함량화mRNA수평급Glu농도균명현강저（F분별위274．620，1121．720；355．870，69．430；294．280，35．330；75．770；P균＜0．001）；동시，간장조직중TNF-α、IL-6화IFN-γmRNA적표체명현강저（F분별위1165．950，69．460，4787．190；P균＜0．001）。결론：HNK대CCl4유도적소서급성간손상유보호작용。
Aim:To observe the protective effect of honokiol on liver injury induced by carbon tetrachloride ( CCl4 ) in mice.Methods:Fifty mice were randomly divided into five groups .The HNK high, middle and low dose groups were injec-ted honokiol solution at a concentration of 20.0, 2.0, and 0.2 mg/kg, respectively, and the normal group and model group were injected normal saline , respectively .After 7 d,animals in the model group and HNK groups were injected 10 mL/kg CCl4 , others were given soybean oil .Histological analysis of liver was performed by hematoxylin and eosin .The contents of serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) and glucose(Glu) were determined through automatic biochemistry analyzer .The mRNA levels of ALT, AST, ALP, tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and interferon-γ(INF-γ) in livers were analyzed by real-time PCR.Results: Compared with the normal group , the liver sections of model group showed destructive alterations in the parenchyma , extensive fatty changes, disarrangement of hepatic cords and central veins , centrilobular necrosis, focal necrosis.The content of ALT, AST, ALP,and Glu in HNK groups were lessened in comparison with control group (F=274.620,1 121.720;355.870, 69.430;294.280,35.330;75.770;P<0.001).The mRNA expression of TNF-α, IL-6 and IFN-γin liver was decreased in comparison with control group(F=1 165.950,69.460,4 787.190;P<0.001)significantly.Conclusion:The HKK can play a protecting role against liver injury induced by CCl 4 .