CAJ | 학술논문

目的：研究大鼠原位肺移植后体内CD8＋调节性T细胞（ CD8＋Treg）的分布及动态变化规律。方法：以cuff技术构建原位左肺移植的Wistar→Lewis大鼠同种肺移植模型20只、Lewis→Lewis同基因移植模型5只以及假手术模型5只，分别在术后不同时间点处死大鼠，切除移植物或者对照肺组织行常规病理学检查，以离心法、研磨法以及裂解法分别获取血液、脾脏、肺门淋巴结以及移植肺内的淋巴细胞，行流式细胞学检查。结果：同种移植组肺内可以见到典型的急性排斥反应。大鼠的血液和组织内检测到了CD8＋CD45 RC-Treg。同基因移植组肺组织及淋巴结内CD8＋CD45RC-Treg/CD3＋和CD8＋CD45RC-Treg/CD8＋分别为（15．88±2．72）％、（13．88±2．81）％和（30．68±5．25）％、（31．43±4．56）％，高于同种移植组（11．27±3．06）％、（9．89±1．86）％和（18．04±4．65）％、（18．89±4．79）％（t＝2．518、2．648和4．030、4．240，P均＜0．05）。结论：原位肺移植后肺组织及淋巴结内出现CD8＋CD45RC-Treg的聚集，排斥反应发生后其在肺内的数量减少。
목적：연구대서원위폐이식후체내CD8＋조절성T세포（ CD8＋Treg）적분포급동태변화규률。방법：이cuff기술구건원위좌폐이식적Wistar→Lewis대서동충폐이식모형20지、Lewis→Lewis동기인이식모형5지이급가수술모형5지，분별재술후불동시간점처사대서，절제이식물혹자대조폐조직행상규병이학검사，이리심법、연마법이급렬해법분별획취혈액、비장、폐문림파결이급이식폐내적림파세포，행류식세포학검사。결과：동충이식조폐내가이견도전형적급성배척반응。대서적혈액화조직내검측도료CD8＋CD45 RC-Treg。동기인이식조폐조직급림파결내CD8＋CD45RC-Treg/CD3＋화CD8＋CD45RC-Treg/CD8＋분별위（15．88±2．72）％、（13．88±2．81）％화（30．68±5．25）％、（31．43±4．56）％，고우동충이식조（11．27±3．06）％、（9．89±1．86）％화（18．04±4．65）％、（18．89±4．79）％（t＝2．518、2．648화4．030、4．240，P균＜0．05）。결론：원위폐이식후폐조직급림파결내출현CD8＋CD45RC-Treg적취집，배척반응발생후기재폐내적수량감소。
Aim: To study the dynamics and distribution of CD 8 +Treg in rat orthotopic lung transplant recipients . Methods:Allogeneic(n=20), isogeneic(n=5) orthotopic lung transplantation by cuff technique or sham operations (n=5) were performed for Lewis recipients .The rats were sacrificed at different postoperative time .Grafts or control lungs were harvested for histological evaluation .Lymphocytes in peripheral blood , spleen and local lymph nodes , and graft were ac-quired by centrifugation , trituration and lysis .Their phenotype and the expression of Foxp 3 were analyzed by flow cytome-try.Results:Typical acute rejections were observed in lung allografts .CD8 +CD45RC-Treg was identified in blood and tissue.CD8+CD45RC-Treg/CD3 +and CD8 +CD45RC-Treg/CD8 +in grafts and hilar lymph nodes of rats from isotrans-plant group were (15.88 ±2.72)%,(13.88 ±2.81)% and (30.68 ±5.25)%,(31.43 ±4.56)%, which were higher than those in allotransplant group [(11.27 ±3.06)%,(9.89 ±1.86)% and (18.04 ±4.65)%,(18.89 ±4.79)%] (t=2.518, 2.648, 4.030, 4.240,P<0.05).Conclusion:CD8 +CD45RC-Treg could aggregate to the grafts and local hilar lymph nodes .Lung Treg decrease in the setting of rejection .