CAJ | 학술논문

目的：探讨阿魏酸（ FA）对大鼠离体胸主动脉环的舒张作用。方法：采用离体血管灌流法，检测FA对去氧肾上腺素（ PE ）和KCl 预收缩大鼠离体胸主动脉环的舒张作用，观察去内皮或L-亚硝基精氨酸甲基酯（ L-NAME）、亚甲蓝（MB）、吲哚美辛（Indo）、格列苯脲（Gli）、四乙基胺（TEA）孵育后舒张作用的变化；并观察在无钙K-H液中FA对PE诱发动脉环收缩作用的影响。结果：FA具有浓度依赖性地舒张PE和KCl预收缩的胸主动环的作用；完整内皮组和去内皮组的舒张作用的pD2分别为2．62±0．08和2．60±0．09（Z＝0．447，P＝0．655），与各种受体阻断剂孵育后FA的舒张作用间差异无统计学意义（P＞0．05）。 FA可明显抑制PE诱发的依外Ca2＋性收缩反应（FFA ＝134．517，P＜0．001）和依内Ca2＋性收缩反应（F＝108．816，P＜0．001）。结论：FA具有舒张动脉环作用，其机制与抑制平滑肌细胞内Ca2＋的释放和外Ca2＋内流有关，其中考虑以抑制细胞内Ca2＋的释放为主。
목적：탐토아위산（ FA）대대서리체흉주동맥배적서장작용。방법：채용리체혈관관류법，검측FA대거양신상선소（ PE ）화KCl 예수축대서리체흉주동맥배적서장작용，관찰거내피혹L-아초기정안산갑기지（ L-NAME）、아갑람（MB）、신타미신（Indo）、격렬분뇨（Gli）、사을기알（TEA）부육후서장작용적변화；병관찰재무개K-H액중FA대PE유발동맥배수축작용적영향。결과：FA구유농도의뢰성지서장PE화KCl예수축적흉주동배적작용；완정내피조화거내피조적서장작용적pD2분별위2．62±0．08화2．60±0．09（Z＝0．447，P＝0．655），여각충수체조단제부육후FA적서장작용간차이무통계학의의（P＞0．05）。 FA가명현억제PE유발적의외Ca2＋성수축반응（FFA ＝134．517，P＜0．001）화의내Ca2＋성수축반응（F＝108．816，P＜0．001）。결론：FA구유서장동맥배작용，기궤제여억제평활기세포내Ca2＋적석방화외Ca2＋내류유관，기중고필이억제세포내Ca2＋적석방위주。
Aim:To investigate the vasodilatation effects and mechanism of ferulic acid ( FA) on isolated thoracic aor-tic rings from rats .Methods: Using isolated vascular methods , the vasodilatation effects of accumulated FA on thoracic aortic rings preconstricted with L-phenylephrine hydrochloride (PE) and KCl were observed.After the isolated thoracic aortic rings were pretreated with removing the endothelium , or pretreated with the drugs , the changes of vasodilatation effects were observed .And in Ca2+-free K-H solution, the influence of FA on thoracic aortic rings proconstricted with PE was observed .Results:With the increasing concentration , FA increased the vasodilatation effects of vascular aortic rings in a dose-dependent manner .The values of pD2 of endothelial groups and the endothelial-denuded group were 2.62 ±0.08, 2.60 ±0.93, respectively (Z=0.447,P=0.655).Compared with them, there was no significant changes after incubation with the drugs of L-NAME, Indo, Gli, TEA, or MB ( P>0.05).In addition, FA inhibited PE-induced contraction in Ca2+-free K-H solution(FFA =134.517,P<0.001).In Ca2+-depleted, PE-treated aortic rings, FA inhibited the Ca2+-in-duced contraction in a concentration-dependent manner (F=108.816,P<0.001).Conclusion: These results indicate that FA relaxes the thoracic aortic rings of rats in an endothelium-indepedended manner , and the vasorelaxing effect of FA might be mediated mainly through inhibiting Ca 2+release from intracellular stores and extracellular Ca 2+influx into vascular smooth muscle cells .