CAJ | 학술논문

目的：探讨胎牛血清（FBS）对人神经母细胞瘤细胞（SH-SY5Y）吗啡戒断后细胞中ERK1/2、p38和JNK磷酸化表达的影响。方法：SH-SY5 Y细胞分为无血清培养组和体积分数为10％血清培养组（有血清培养组）。细胞经10μmol/L吗啡作用48 h后，10μmol/L纳洛酮戒断。在纳洛酮戒断不同时间后，收集细胞，应用Western blot检测ERK1/2、JNK和p38蛋白磷酸化水平的变化。结果：无血清培养条件下纳洛酮戒断10 min后SH-SY5Y细胞中ERK1/2磷酸化水平显著上调（F＝6．619，P＜0．001），戒断30、60 min后ERK1/2磷酸化水平与对照组差异无统计学意义。有血清培养条件下纳洛酮戒断10、30和60 min后SH-SY5Y细胞中ERK1/2磷酸化水平均显著上调（F＝22．901，P＜0．001）；在无和有血清培养条件下纳洛酮戒断均引起SH-SY5Y细胞中p38磷酸化水平呈时间依赖性递增，同时也能引起JNK磷酸化水平的增加，但不随戒断时间的增加而升高。结论：SH-SY5Y细胞在无血清培养下，吗啡戒断时ERK1/2磷酸化为瞬时增高；血清培养下，吗啡戒断可诱导ERK1/2磷酸化持续增高。
목적：탐토태우혈청（FBS）대인신경모세포류세포（SH-SY5Y）마배계단후세포중ERK1/2、p38화JNK린산화표체적영향。방법：SH-SY5 Y세포분위무혈청배양조화체적분수위10％혈청배양조（유혈청배양조）。세포경10μmol/L마배작용48 h후，10μmol/L납락동계단。재납락동계단불동시간후，수집세포，응용Western blot검측ERK1/2、JNK화p38단백린산화수평적변화。결과：무혈청배양조건하납락동계단10 min후SH-SY5Y세포중ERK1/2린산화수평현저상조（F＝6．619，P＜0．001），계단30、60 min후ERK1/2린산화수평여대조조차이무통계학의의。유혈청배양조건하납락동계단10、30화60 min후SH-SY5Y세포중ERK1/2린산화수평균현저상조（F＝22．901，P＜0．001）；재무화유혈청배양조건하납락동계단균인기SH-SY5Y세포중p38린산화수평정시간의뢰성체증，동시야능인기JNK린산화수평적증가，단불수계단시간적증가이승고。결론：SH-SY5Y세포재무혈청배양하，마배계단시ERK1/2린산화위순시증고；혈청배양하，마배계단가유도ERK1/2린산화지속증고。
Aim:To investigate the effects of fetal bovine serum (FBS) on phosphorylated ERK1/2, p38 and JNK in human neuroblastoma cells ( SH-SY5Y) during morphine withdrawal .Methods:SH-SY5Y cells were cultured either in DMEM/F12(1:1) without serum or in DMEM/F12(1:1) with serum (10% FBS).All cells were treated with 10μol/L morphine for 48 h.Cells were harvested at different time after 10 μol/L naloxone was added .Western blot was employed to detect the phosphorylation of ERK 1/2, p38 and JNK.Results:ERK1/2 phosphorylation was significantly in-creased 10 min after naloxone-precipitated morphine withdrawal in SH-SY5Y cells cultured without FBS (F=6.619, P<0.001).However, this increase disappeared after 30 min, 60 min.On the contrary, naloxone-precipitated morphine with-drawal induced sustained ERK1/2 phosphorylation in SH-SY5Y cells cultured with 10%FBS (F=22.901, P<0.001). In contrast, naloxone-precipitated morphine withdrawal induced similar p 38 and JNK phosphorylation increase in SH-SY5Y cells cultured with or without FBS .Conclusion:Morphine withdrawal induces instant increase of ERK 1/2 phosphorylation when cultured without FBS while sustained increase of ERK 1/2 phosphorylation in SH-SY5Y cells cultured with FBS .The temporal pattern of JNK and p38 phospholyration is similar in SH-SY5Y cells cultured with or without FBS .