CAJ | 학술논문

目的：对豫医卷毛大鼠的表皮生长因子（ EGF）基因进行比较生物学分析。方法：采用PCR方法对豫医卷毛大鼠EGF基因cDNA序列进行克隆测序，并与BN大鼠、家鼠、原鸡、猕猴和人EGF基因的核苷酸序列及其编码蛋白的氨基酸序列进行同源性比较和系统遗传学分析。结果：获得了豫医卷毛大鼠EGF基因全长3522 bp的cDNA序列（ GenBank登录号：JX437943）。豫医卷毛大鼠EGF基因编码区的核苷酸序列与BN大鼠、家鼠、原鸡、猕猴和人的同源性分别为97．8％、83．4％、59．7％、76．5％、73．5％，其编码蛋白的氨基酸序列同源性分别为99．3％、79．9％、52．9％、69．6％、69．4％。结论：EGF基因在进化过程中具有一定的保守性，但不同物种之间也具有特异性。
목적：대예의권모대서적표피생장인자（ EGF）기인진행비교생물학분석。방법：채용PCR방법대예의권모대서EGF기인cDNA서렬진행극륭측서，병여BN대서、가서、원계、미후화인EGF기인적핵감산서렬급기편마단백적안기산서렬진행동원성비교화계통유전학분석。결과：획득료예의권모대서EGF기인전장3522 bp적cDNA서렬（ GenBank등록호：JX437943）。예의권모대서EGF기인편마구적핵감산서렬여BN대서、가서、원계、미후화인적동원성분별위97．8％、83．4％、59．7％、76．5％、73．5％，기편마단백적안기산서렬동원성분별위99．3％、79．9％、52．9％、69．6％、69．4％。결론：EGF기인재진화과정중구유일정적보수성，단불동물충지간야구유특이성。
Aim:To study the difference of epidermal growth factor ( EGF) gene and its coding protein between Yuyi curly hair rats and others species .Methods:PCR technique was developed to clone the sequence of cDNA of EGF gene from Yuyi curly hair rats , and biologic characters of nucleotide and amino acid sequence encoding EGF gene in different species ( Norway rats , house mice, chicken , rhesus monkey, and human ) were analyzed .Results:A full-length cDNA of Yuyi curly hair rats was 3 522 bp ( Accession Number:JX437943 ) .The EGF gene nucleotide homology of Yuyi curly hair rats to Norway rats, house mice, chicken, rhesus monkey, and human was 97.8%, 83.4%, 59.7%, 76.5%, and 73.5%, and that of amino acid sequences was99.3%,79.9%,52.9%,69.6%,and 69.4%, respectively.Conclusion:A certain degree of conservation of EGF gene exists among different mammalian taxons , but there were some specificity a-mong different mammalian taxons .