中国妇幼健康研究
中國婦幼健康研究
중국부유건강연구
CHINESE JOURNAL OF MATERNAL AND CHILD HEALTH RESEARCH
2013年
4期
492-494
,共3页
刘昳%宗时春%叶峰%师娟子%金燕%蔺淑梅%孔颖%张曦%杨雪亮%张健
劉昳%宗時春%葉峰%師娟子%金燕%藺淑梅%孔穎%張晞%楊雪亮%張健
류질%종시춘%협봉%사연자%금연%린숙매%공영%장희%양설량%장건
乙型肝炎病毒%胚胎%乙型肝炎病毒信使RNA%母婴垂直传播
乙型肝炎病毒%胚胎%乙型肝炎病毒信使RNA%母嬰垂直傳播
을형간염병독%배태%을형간염병독신사RNA%모영수직전파
HBV%embryo%HBV mRNA%vertical tranmission
目的通过对乙型肝炎病毒（ HBV）感染母亲卵细胞的体外受精胚胎中HBV mRNA的检测，明确HBV是否能够经卵细胞传递至胚胎而发生母婴垂直传播。方法以慢性HBV感染母亲卵细胞体外受精后遗弃胚胎这一特殊标本为研究对象，用单细胞RT-PCR的方法检测胚胎中的HBV mRNA。结果38例HBV血清标志物阳性母亲中有5例胚胎中出现阳性，阳性率为13．2％（5/38），其中5个废弃胚胎中有3个出现阳性信号，母亲血清HBeAg阳性，HBVDNA定量为4．7×105 IU/mL；另有3个废弃胚胎中有2个检出阳性信号，母亲血清HBeAg阴性，HBVDNA定量≤103 IU/mL。结论 HBV能够经卵细胞传递至胚胎而发生母婴垂直传播，并在其中复制。
目的通過對乙型肝炎病毒（ HBV）感染母親卵細胞的體外受精胚胎中HBV mRNA的檢測，明確HBV是否能夠經卵細胞傳遞至胚胎而髮生母嬰垂直傳播。方法以慢性HBV感染母親卵細胞體外受精後遺棄胚胎這一特殊標本為研究對象，用單細胞RT-PCR的方法檢測胚胎中的HBV mRNA。結果38例HBV血清標誌物暘性母親中有5例胚胎中齣現暘性，暘性率為13．2％（5/38），其中5箇廢棄胚胎中有3箇齣現暘性信號，母親血清HBeAg暘性，HBVDNA定量為4．7×105 IU/mL；另有3箇廢棄胚胎中有2箇檢齣暘性信號，母親血清HBeAg陰性，HBVDNA定量≤103 IU/mL。結論 HBV能夠經卵細胞傳遞至胚胎而髮生母嬰垂直傳播，併在其中複製。
목적통과대을형간염병독（ HBV）감염모친란세포적체외수정배태중HBV mRNA적검측，명학HBV시부능구경란세포전체지배태이발생모영수직전파。방법이만성HBV감염모친란세포체외수정후유기배태저일특수표본위연구대상，용단세포RT-PCR적방법검측배태중적HBV mRNA。결과38례HBV혈청표지물양성모친중유5례배태중출현양성，양성솔위13．2％（5/38），기중5개폐기배태중유3개출현양성신호，모친혈청HBeAg양성，HBVDNA정량위4．7×105 IU/mL；령유3개폐기배태중유2개검출양성신호，모친혈청HBeAg음성，HBVDNA정량≤103 IU/mL。결론 HBV능구경란세포전체지배태이발생모영수직전파，병재기중복제。
Objective To confirm whether vertical transmission of hepatitis B virus ( HBV) can occur via infected ovum by detecting HBV mRNA in the test-tube embryos of mothers with chronic HBV .Methods Specimens were discarded test-tube embryos from mothers with chronic HBV infection who had received in vitro fertilization treatment .Single-cell reverse transcriptase-polymerase chain reaction ( RT-PCR) was used to detect HBV mRNA in the embryos .Results HBV mRNA was detected in the cleavage embryos of 5 cases with chronic HBV infection, with a detection rate of 13.2%(5/38), including 3 embryos of the 5 discarded ones detected with positive signal .Maternal HBeAg was positive, and quantitative HBVDNA was 4.7 ×105IU/mL.Positive signal was detected in 2 of another 3 discarded embryos. Maternal HBeAg was negative with quantitative HBVDNA≤103 IU/mL.Conclusion HBV can be transferred to embryo via ovum , and vertical transmission is confirmed .Meanwhile , HBV is duplicated in embryo .
