CAJ | 학술논문

目的分析反复呼吸道感染患儿细胞及体液免疫功能。方法选择2010年10月至2012年9月确诊为反复呼吸道感染患儿60例，按照年龄将其分为：A1组年龄＜3岁，B1组3～6岁；同时选择健康儿童60例作为对照组：A2组年龄＜3岁，B2组3～6岁。采用流式细胞术检测所有入组儿童外周血T淋巴细胞总数（ CD3＋）及其亚群（ CD3＋CD4＋、CD3＋CD8＋）的绝对计数；速率散射比浊法测定其血清免疫球蛋白G、A、M（ IgG、IgA、IgM）含量。结果 A1、B1组CD3＋、CD3＋CD4＋细胞绝对计数及IgG含量均较相应正常对照组A2、B2组降低，差异均有统计学意义（A1与A2组比较t值分别为0．003、0．033、0．017；B1与B2组比较t值分别为0．041、0．045、0．012，均P＜0．05）。在＜3岁儿童中，反复呼吸道感染患儿的IgA含量较正常同龄儿童偏低，差异有统计学意义（t＝0．035，P＜0．05）。结论反复呼吸道感染患儿CD3＋T淋巴细胞总数绝对计数的降低，尤其是CD3＋CD4＋T辅助细胞的减少，使其对T、B淋巴细胞增殖分化的调控作用削弱，浆细胞产生Ig过程障碍是导致儿童反复呼吸道感染的重要原因。
목적분석반복호흡도감염환인세포급체액면역공능。방법선택2010년10월지2012년9월학진위반복호흡도감염환인60례，안조년령장기분위：A1조년령＜3세，B1조3～6세；동시선택건강인동60례작위대조조：A2조년령＜3세，B2조3～6세。채용류식세포술검측소유입조인동외주혈T림파세포총수（ CD3＋）급기아군（ CD3＋CD4＋、CD3＋CD8＋）적절대계수；속솔산사비탁법측정기혈청면역구단백G、A、M（ IgG、IgA、IgM）함량。결과 A1、B1조CD3＋、CD3＋CD4＋세포절대계수급IgG함량균교상응정상대조조A2、B2조강저，차이균유통계학의의（A1여A2조비교t치분별위0．003、0．033、0．017；B1여B2조비교t치분별위0．041、0．045、0．012，균P＜0．05）。재＜3세인동중，반복호흡도감염환인적IgA함량교정상동령인동편저，차이유통계학의의（t＝0．035，P＜0．05）。결론반복호흡도감염환인CD3＋T림파세포총수절대계수적강저，우기시CD3＋CD4＋T보조세포적감소，사기대T、B림파세포증식분화적조공작용삭약，장세포산생Ig과정장애시도치인동반복호흡도감염적중요원인。
Objective To analyze the cellular and humoral immunity of children affected by recurrent respiratory tract infections ( RRTI) . Methods Sixty children diagnosed with RRTI during the period of October 2010 to September 2012 were divided into A1 group(<3 years of age) and B1 group(3-6 years of age).Meanwhile, 60 healthy children were taken as control group:A2 group(<3 years of age) and B2 group(3-6 years of age).The absolute count of CD3+T lymphocyte as well as CD3+CD4+and CD3+CD8+subsets of enrolled children were detected by flow cytometry , and the serum content of immunoglobulin G , A and M ( IgG, IgA and IgM ) was also determined by rate nephelometry.Results The absolute counts of CD3+and CD3+CD4+and IgG content both in A1 and B1 group were observed to be lower than the corresponding counts and content in A 2 and B2 group, and there were statistical differences between patients and control groups (comparing A1 with A2 group, t value was 0.003, 0.033 and 0.017, respectively;comparing B1 with B2 group, t value was 0.041, 0.045 and 0.012, respectively, all P<0.05).When children were under 3 years old, those with RRTI had lower content of IgA than healthy children with same age, and the difference was significant(t=0.035, P<0.05).Conclusion The decrease of absolute count of CD3+T lymphocyte, especially the decrease of CD3+CD4+T helper cell, weakens the regulatory function on proliferation and differentiation of T and B lymphocyte , which leads to obstruction of Ig molecules production .That is the important reason for children RRTI .