CAJ | 학술논문

为探究胃瘤线虫幼虫体外存活影响因素，观察了黄鳝源胃瘤线虫幼虫在不同温度、溶液、pH值条件下的存活情况。结果表明：胃瘤线虫幼虫在-36℃、-20℃、-7℃的存活时间分别为（2±0.71） h、（5±1.15）h、（5±1.15）d；在4℃、16℃~28℃、32℃及68℃的存活时间分别为（55±5.29）d、9 d以上、3 d及(5±2) s。胃瘤线虫幼虫在pH=7.4、pH=7、pH≥8.87或pH≤5.10溶液中，存活时间分别为（15±2）d、（8±1）d、（2±1）d。胃瘤线虫幼虫在2%葡萄糖溶液、任氏液、0.9%生理盐水、蒸馏水、高浓度（≥6.5%）NaCl溶液中存活时间分别为（14±2）d、（11.67±1.53）d、（10.33±3.21）d、（8±1）d、（4.33±0.58）h。本试验结果可为胃瘤线虫幼虫的实验室保存及肉品安全参数提供依据。
위탐구위류선충유충체외존활영향인소，관찰료황선원위류선충유충재불동온도、용액、pH치조건하적존활정황。결과표명：위류선충유충재-36℃、-20℃、-7℃적존활시간분별위（2±0.71） h、（5±1.15）h、（5±1.15）d；재4℃、16℃~28℃、32℃급68℃적존활시간분별위（55±5.29）d、9 d이상、3 d급(5±2) s。위류선충유충재pH=7.4、pH=7、pH≥8.87혹pH≤5.10용액중，존활시간분별위（15±2）d、（8±1）d、（2±1）d。위류선충유충재2%포도당용액、임씨액、0.9%생리염수、증류수、고농도（≥6.5%）NaCl용액중존활시간분별위（14±2）d、（11.67±1.53）d、（10.33±3.21）d、（8±1）d、（4.33±0.58）h。본시험결과가위위류선충유충적실험실보존급육품안전삼수제공의거。
In vitro survival time of eustrongylides was evaluated in the present study when stored at different temperature, pH and preservation solutions. The viability of eustrongylides lasted (2±0.71) h, (5±1.15) h and (5±1.15) d at-36℃,-20℃and-7℃, or (55±5.29) d,>9 d , 3 d and (5±2) s at 4℃, 16℃-28℃, 32℃and 68℃. When pH was 7.4, 7, ≥8.87 or ≤5.10, eustrongylides survived for (15±2) d, (8±1) d or (2±1) d. Eustrongylides kept viability for (14±2) d, (11.67±1.53) d, (10.33±3.21) d, (8±1) d and (4.33±0.58) h in 2%amylaceum, Ringer’s fluid, 0.9%NaCl, distilled water and high concentration of NaCl (≥6.5%). These results provided useful information for the preservation of eustrongylides in laboratory and for food safety.