CAJ | 학술논문

为了调查新疆地区规模化奶牛场牛支原体的感染情况，采用牛支原体间接ELISA和特异性PCR检测牛血清中的牛支原体抗体及病料中牛支原体核酸，共检测9个地区15个规模化奶牛场437份血清，肺脏、关节液及鼻腔黏液44份。结果显示，血清抗体阳性率为76.43%（334/437），其中脐带血抗体阳性率为40.00%（4/10）；病料阳性率为40.91%（18/44）。检测结果表明，新疆地区大部分奶牛场存在牛支原体感染，部分奶牛场发生牛支原体肺炎及关节炎病例，并存在垂直传播的风险。牛支原体感染可能成为危害新疆规模化奶牛场犊牛健康的主要疫病之一。
위료조사신강지구규모화내우장우지원체적감염정황，채용우지원체간접ELISA화특이성PCR검측우혈청중적우지원체항체급병료중우지원체핵산，공검측9개지구15개규모화내우장437빈혈청，폐장、관절액급비강점액44빈。결과현시，혈청항체양성솔위76.43%（334/437），기중제대혈항체양성솔위40.00%（4/10）；병료양성솔위40.91%（18/44）。검측결과표명，신강지구대부분내우장존재우지원체감염，부분내우장발생우지원체폐염급관절염병례，병존재수직전파적풍험。우지원체감염가능성위위해신강규모화내우장독우건강적주요역병지일。
The present study was undertaken to investigate into Mycoplasma bovis infection on large-scale dairy farms in Xinjiang. Total 427 serum samples, 10 umbilical cord blood samples and 44 diseased tissue samples were collected from 15 large-scale dairy farms located in 9 agriculture and animal husbandry divisions. These samples were examined using indirect ELISA and PCR to determine the presence of Mycoplasmabovis antibodies and/or nucleic acid materials. The positive rates in serum, umbilical cord blood, diseased tissue samples were determined to be 78.45%(335/427), 40.00%(4/10) and 40.91%(18/44), respectively. These results indicated the prevalence of Mycoplasmabovis infection on dairy farms. Some dairy cows suffered from pneumonia and arthritis caused by Mycoplasmabovis and vertical transmission might play a role. These findings provided suggesfed that Mycoplasmabovis infection was prevalent in dairy cow population and might make a major factor for economic loss of dairy farmers in Xinjiang.