CAJ | 학술논문

结合基因功能分类体系(Gene Ontology，GO)对筛选得到的玉米穗粒腐病差异表达基因片段进行全面生物学功能注释。以抗玉米穗粒腐病的自交系Bt-1为材料，接种串珠镰刀菌(Fusarium moniliforme)，对接菌后96 h的玉米苞叶样品以玉米全基因组基因芯片进行大规模筛选，利用分子注释系统(Molecular Annotation System，MAS)对筛选得到的基因片段进行GO分子功能注释，分析特征基因的功能。结果表明，利用基因芯片从抗性材料Bt-1中获得的482条差异代表序列，GO注释获得大量重要信息，对应的基因注释包括分子功能、生物过程和细胞组分3个层次。GO能够准确预测与玉米穗粒腐病相关抗病基因的功能，对于了解表达基因之间的相互关系和深入挖掘、理解高通量数据等方面的具有重大帮助，是研究抗病基因不可或缺的生物学信息手段。
결합기인공능분류체계(Gene Ontology，GO)대사선득도적옥미수립부병차이표체기인편단진행전면생물학공능주석。이항옥미수립부병적자교계Bt-1위재료，접충천주렴도균(Fusarium moniliforme)，대접균후96 h적옥미포협양품이옥미전기인조기인심편진행대규모사선，이용분자주석계통(Molecular Annotation System，MAS)대사선득도적기인편단진행GO분자공능주석，분석특정기인적공능。결과표명，이용기인심편종항성재료Bt-1중획득적482조차이대표서렬，GO주석획득대량중요신식，대응적기인주석포괄분자공능、생물과정화세포조분3개층차。GO능구준학예측여옥미수립부병상관항병기인적공능，대우료해표체기인지간적상호관계화심입알굴、리해고통량수거등방면적구유중대방조，시연구항병기인불가혹결적생물학신식수단。
By Gene ontology(GO), the information of differentially expressed genes after Fusarium moniliforme (FM) infection in maize ear rot was studied. Maize inbred line Bt-1 was use in the experimental material. After inocu-lating at 96h with FM, treated samples and mock-inoculation controls were investigated by using the genechip maize genome array for hybridization. Each of differentially expressed genes was deeply analyzed by gene ontology annota-tion. In total, there were 482 unique genes differentially expressed more than 1.5 fold in inbred Bt-1 when compared to mock-inoculated bract tissues from genechip data. Many genes identified by genechip were associated with roles in disease defense for its direct or indirect antifungal activities in response to FM infection. These large responsive genes with significant differentially change were sorted into cellular component, molecular function and biological process with complicated network by molecular annotation system analysis. Altogether, the present Gene ontology analysis provides another view for understanding the molecular mechanism and providing new insights into the deeply compli-cated network with gene function underlying disease development in the host tissue.