CAJ | 학술논문

本文将乙型脑炎病毒（Japanese encephalitis virus, JEV）基因I型HEN0701株在BHK-21细胞上传代，并通过空斑纯化和接种小鼠来筛选弱毒株。通过空斑试验和一步生长曲线，比较弱毒株和其亲本强毒株的生长特性。结果显示，JEV HEN0701株经过在BHK-21细胞上传代100代和10轮空斑纯化，挑选获得6株克隆株。将6株克隆株经脑内和皮下接种小鼠，筛选获得一株致弱株10S3。与HEN0701相比，10S3的空斑形态比较一致；而生长曲线显示，HEN0701在BHK-21上生长速度比10S3快。
본문장을형뇌염병독（Japanese encephalitis virus, JEV）기인I형HEN0701주재BHK-21세포상전대，병통과공반순화화접충소서래사선약독주。통과공반시험화일보생장곡선，비교약독주화기친본강독주적생장특성。결과현시，JEV HEN0701주경과재BHK-21세포상전대100대화10륜공반순화，도선획득6주극륭주。장6주극륭주경뇌내화피하접충소서，사선획득일주치약주10S3。여HEN0701상비，10S3적공반형태비교일치；이생장곡선현시，HEN0701재BHK-21상생장속도비10S3쾌。
The genotype I (GI) strain HEN0701 of Japanese encephalitis virus was serially passaged up to 100 times and plaque cloned 10 times in BHK-21 cells. The attenuated strain 10S3 was identified via pathogenecity tests in mice. With a little difference in plaque size, the 10S3 strain showed more uniform plaque morphology than its parent HEN0701. The growth kinetic of the 10S3 strain was different from that of HEN0701.