CAJ | 학술논문

对红松松塔多糖的膜分离制备工艺进行研究。采用微滤、超滤联用的方法制备红松松塔多糖，考察了微滤过程中料液温度、操作压力和料液浓度对膜通量的影响，并通过正交实验确定了最佳微滤条件；利用超滤对红松松塔多糖料液进行纯化，并用截留分子量100kDa、50 kDa、10 kDa、6kDa的超滤膜对红松松塔多糖进行了分级分离。结果表明，微滤最佳条件为料液浓度25g/L、压力0．10M Pa、温度40℃；超滤纯化后多糖含量由7．20％提高到35．16％。得出红松松塔多糖分子量分布结果如下：分子量＞10万的占9．50％，5万～10万之间的占70．20％，1万～5万之间的占3．20％，6000～1万之间的占17．1％。结论，应用膜分离制备红松松塔多糖可行，且能较好地分离纯化红松松塔多糖。
대홍송송탑다당적막분리제비공예진행연구。채용미려、초려련용적방법제비홍송송탑다당，고찰료미려과정중료액온도、조작압력화료액농도대막통량적영향，병통과정교실험학정료최가미려조건；이용초려대홍송송탑다당료액진행순화，병용절류분자량100kDa、50 kDa、10 kDa、6kDa적초려막대홍송송탑다당진행료분급분리。결과표명，미려최가조건위료액농도25g/L、압력0．10M Pa、온도40℃；초려순화후다당함량유7．20％제고도35．16％。득출홍송송탑다당분자량분포결과여하：분자량＞10만적점9．50％，5만～10만지간적점70．20％，1만～5만지간적점3．20％，6000～1만지간적점17．1％。결론，응용막분리제비홍송송탑다당가행，차능교호지분리순화홍송송탑다당。
Objective To study the memberane separation technology of preparating polysaccharide of Pinus koraiensis corns .Methods Microfiltration (MF) and ultrafiltration (UF) was used to preparate polysaccharide of Pinus koraiensis corns .Effects of concentration ,temperature ,pressure of feed on exudate flux of MF was studied ,and the best condition was found by orthogonal test . Membrane ,molecular cut off was 100 ,50 ,10 and 6kDa ,was used to separated polysaccharide of Pinus koraiensis corns .Results The best condition of MF was 25g/L ,40 ℃ ,0 .10 MPa .Ultrafil-tration purified polysaccharide content increased from 7 .20% to 35 .16% .The pine cone polysac-charide molecular weight distribution ,the results are as follows :Polysaccharide with molecular weight >100 kDa content accounting for 9 .50% ,50-100 kDa content accounting for 70 .20% ,10-50 kDa content accounting for 3 .20% .6-10 kDa content accounting for 17 .1% ,Conclusion Mem-brane separation technology can be used to preparate and purify polysaccharide of Pinus koraien-sis corns .