CAJ | 학술논문

目的探讨初乳HBV-DNA定量检测对乙肝产妇哺乳的指导意义.方法选择住院分娩产妇325例,依据乙肝五项血清学指标分为五组感染模式.其中A组(65例):乙肝HB、HBeAg和HBcAb均为阳性;B组(9例):乙肝HBsAg、HBeAg、HBeAb和HBcAb均为阳性;C组(152例):乙肝HBsAg、HBeAb和HBcAb均为阳性;D组(47例):乙肝HBsAg、HBcAb为阳性;E组(52例):乙肝五项指标全阴性为对照组.采用化学发光法检测产妇HBV标志物,同时采用实时荧光定量PCR检测产妇血清和初乳HBV-DNA载量,对相关数据进行组间卡方检验分析.结果 A组血清和乳汁HBV-DNA阳性率分别为100.00％(65/65)和83.08％ (54/65),乳汁HBV-DNA载量平均为3.82 ×104copies·ml-1;B组血清和乳汁HBV-DNA阳性率分别为100.00％ (9/9)和77.78％(7/9),乳汁HBV-DNA载量平均为1.26×104copies·ml-1;C组血清和乳汁HBV-DNA阳性率分别为17.10％(26/152)和1.97％ (3/152),乳汁HBV-DNA载量平均为2.31×103copies·ml-1;D组血清和乳汁HBV-DNA阳性率分别为44.68％(21/47)和23.40％(11/47),乳汁HBV-DNA载量平均为6.17×103copies·ml-1;E组血清和乳汁HBV-DNA阳性率均为0(0/52),乳汁HBV-DNA载量为＜1.0×103copies·ml-1.A组与B组乳汁HBV-DNA阳性率(83.08％、77.78％)明显高于C组(1.97％),差异有统计学意义(x2值分别为135.26和115.31,P ＜0.05);产妇乳汁HBV-DNA与血清中HBV-DNA水平呈正相关.结论血清乙肝免疫学检测呈HBsAg和HBeAg双阳性的产妇不宜哺乳,血清呈HBsAg、HBeAb和HBcAb阳性但同时乳汁HBV-DNA阴性者宜谨慎哺乳.
목적 탐토초유HBV-DNA정량검측대을간산부포유적지도의의.방법 선택주원분면산부325례,의거을간오항혈청학지표분위오조감염모식.기중A조(65례):을간HB、HBeAg화HBcAb균위양성;B조(9례):을간HBsAg、HBeAg、HBeAb화HBcAb균위양성;C조(152례):을간HBsAg、HBeAb화HBcAb균위양성;D조(47례):을간HBsAg、HBcAb위양성;E조(52례):을간오항지표전음성위대조조.채용화학발광법검측산부HBV표지물,동시채용실시형광정량PCR검측산부혈청화초유HBV-DNA재량,대상관수거진행조간잡방검험분석.결과 A조혈청화유즙HBV-DNA양성솔분별위100.00％(65/65)화83.08％ (54/65),유즙HBV-DNA재량평균위3.82 ×104copies·ml-1;B조혈청화유즙HBV-DNA양성솔분별위100.00％ (9/9)화77.78％(7/9),유즙HBV-DNA재량평균위1.26×104copies·ml-1;C조혈청화유즙HBV-DNA양성솔분별위17.10％(26/152)화1.97％ (3/152),유즙HBV-DNA재량평균위2.31×103copies·ml-1;D조혈청화유즙HBV-DNA양성솔분별위44.68％(21/47)화23.40％(11/47),유즙HBV-DNA재량평균위6.17×103copies·ml-1;E조혈청화유즙HBV-DNA양성솔균위0(0/52),유즙HBV-DNA재량위＜1.0×103copies·ml-1.A조여B조유즙HBV-DNA양성솔(83.08％、77.78％)명현고우C조(1.97％),차이유통계학의의(x2치분별위135.26화115.31,P ＜0.05);산부유즙HBV-DNA여혈청중HBV-DNA수평정정상관.결론 혈청을간면역학검측정HBsAg화HBeAg쌍양성적산부불의포유,혈청정HBsAg、HBeAb화HBcAb양성단동시유즙HBV-DNA음성자의근신포유.
Objective The value of colostrum HBV DNA quantities among HBV-positive lying-in Women Methods 325 lying-in women were selected according to the principle of inform consent.These women were classified into 5 groups.Group A:65 lying-in women with strong-infectious HBV (three positives),group B:9 lying-in women with strong-infectious HBV (four positives),group C:152 lying-in women with weak-infectious HBV (three positives),group D:47 lying-in women with the first and the fifth positive HBV,group E:52 HBV-negative lying-in women.The HBV markers in the blood serum and colostrum of selected women were detected by Chemiluminescence (CL).Furthermore PCR was utilized to measure the serum HBV DNA quantities and colostrum HBV DNA quantities from the lying-in women.The data was analyzed after been collected.Results The blood serum and colostrum HBV DNA positive-rate among the lying-in women in group A respectively were 100.00％ (65/65)and 83.08％ (54/65),and the average colostrum HBV DNA quantity was 3.82 × 104copies ml-1.The blood serum and colostrum HBV DNA positive-rate among the lying-in women in group B respectively were 100％ (9/9) and 77.78％ (7/9),and the average colostrum HBV DNA quantity was 1.26 × 104copies ml-1.The blood serum and colostrum HBV DNA positive-rate among the lying-in women in group C respectively were 17.10％ (26/152) and 1.97％ (3/152),and the average colostrum HBV DNA quantity was 2.31 × 103copies ml-1.The blood serum and colostrum HBV DNA positive-rate among the lying-in women in group D respectively were 44.68％ (21/47) and 23.40％ (11/47),and the average colostrum HBV DNA quantity was 6.17 × 103copies ml-1.The blood serum and colostrum HBV DNA positive-rate among the lying-in women in group E respeetively were 0 (0/52) and 0(0/52) and the average colostrum HBV DNA quantity was ＜ 1.0 × 103copies ml-1.There were significant difference in positive individuals which were detected by colostrum HBV DNA experiments between group A,B and C (x2 =135.26 and 115.31,P ＜0.01).The colostrum infectivity of the HBV-positive persons corrected with their mothers' bloods.Conclusions It isn' t safe for lying-in women with strong-infectious HBV (HBsAg and HBeAg positives) to feed.It is relatively safe for lying-in women with weak-infectious HBV (HBsAg,HBeAb and HBcAb positives) to feed.