CAJ | 학술논문

探讨4周自愿转轮运动对慢性不可预知性应激致抑郁大鼠旷场行为及空间学习记忆能力、血清皮质醇和基底外侧杏仁核c-fos 表达的影响。将40只大鼠随机分为4组，即对照组、运动组、应激模型组及应激运动组，每组10只。应激模型组及应激运动组大鼠每日进行慢性不可预知性应激1次，连续28 d，同时运动组及应激运动组大鼠进行4周自愿转轮运动。自愿转轮运动结束后检测大鼠血清皮质醇水平；运用旷场实验测试大鼠自主活动能力及探索行为；八臂迷宫实验检测大鼠空间学习记忆能力；采用免疫组织化学结合图像半定量方法对基底外侧杏仁核 c-fos神经元的数量、面积及灰度进行测量和分析。结果发现：1)与应激模型组比较，应激运动组大鼠穿越格数、直立次数及修饰次数显著增多(P<0.01、P<0.05)，粪便颗粒显著减少(P<0.05)；2)与应激模型组比较，应激运动组大鼠血清皮质醇水平显著下降(P<0.05)，完成八臂迷宫时间显著缩短(P<0.01)，WME、RME显著减少(P<0.05)，总错误次数(TE)远远低于应激模型组(P<0.01)；3)与应激模型组比较，应激运动组基底外侧杏仁核 c-fos 免疫阳性细胞数量和阳性产物面积表达均增加(P<0.05)，阳性细胞灰度值虽降低，但没有显著差异性(P>0.05)。以上结果说明，自愿转轮运动可提高抑郁大鼠的学习记忆能力，机理可能与长期自愿转轮运动降低抑郁大鼠的血清皮质醇水平及增强大脑基底外侧杏仁核c-fos 的表达有关。
탐토4주자원전륜운동대만성불가예지성응격치억욱대서광장행위급공간학습기억능력、혈청피질순화기저외측행인핵c-fos 표체적영향。장40지대서수궤분위4조，즉대조조、운동조、응격모형조급응격운동조，매조10지。응격모형조급응격운동조대서매일진행만성불가예지성응격1차，련속28 d，동시운동조급응격운동조대서진행4주자원전륜운동。자원전륜운동결속후검측대서혈청피질순수평；운용광장실험측시대서자주활동능력급탐색행위；팔비미궁실험검측대서공간학습기억능력；채용면역조직화학결합도상반정량방법대기저외측행인핵 c-fos신경원적수량、면적급회도진행측량화분석。결과발현：1)여응격모형조비교，응격운동조대서천월격수、직립차수급수식차수현저증다(P<0.01、P<0.05)，분편과립현저감소(P<0.05)；2)여응격모형조비교，응격운동조대서혈청피질순수평현저하강(P<0.05)，완성팔비미궁시간현저축단(P<0.01)，WME、RME현저감소(P<0.05)，총착오차수(TE)원원저우응격모형조(P<0.01)；3)여응격모형조비교，응격운동조기저외측행인핵 c-fos 면역양성세포수량화양성산물면적표체균증가(P<0.05)，양성세포회도치수강저，단몰유현저차이성(P>0.05)。이상결과설명，자원전륜운동가제고억욱대서적학습기억능력，궤리가능여장기자원전륜운동강저억욱대서적혈청피질순수평급증강대뇌기저외측행인핵c-fos 적표체유관。
In order to probe into the effects of 4-week voluntary wheeling exercise on the open field behavior, space learning and memorizing ability, blood serum cortisol and c-fos expression of basolateral amygdaloid nucleus of rats having depression caused by chronic and unpredictable stress, the authors divided 40 rats randomly into 4 groups:a control group, an exercise group, a stress model group and a stress exercise group, each of which included 10 rats, put the rats in the stress model group and stress exercise group under chronic and unpredictable stress once a day for consecutive 28 d, let the rats in the exercise group and stress exercise group do a voluntary wheeling exercise for 4 weeks, measured the level of blood serum cortisol of the rats after the voluntary wheeling exercise was completed, applied the open field experiment to test the automatic movement ability and explorative behavior of the rats, ap-plied the 8-arm radial maze experiment to measure the space learning and memorizing ability of the rats, applied immunohistochemical and image semi-quantitative methods to measure and analyze the number, area and grey level of c-fos neurons of basolateral amygdaloid nucleus, and revealed the following findings:1) as compared with the rats in the stress model group, the rats in the stress exercise group had a significantly increased number of squares crossed, number of times of standing up, number of times of decoration (P<0.01, P<0.05), and a significantly de-creased number of fecal granules (P<0.05);2) as compared with the rats in the stress model group, the rats in the stress exercise group had a significantly decreased level of blood serum cortisol (P<0.05), a significantly shortened time to complete the 8-arm radial maze (P<0.01), significantly reduced WME and RME (P<0.05), and a much smaller number of total errors (P<0.01);3) as compared with the rats in the stress model group, the rats in the stress exercise group had an increased number of c-fos immune positive cells of basolateral amygdaloid nucleus and an increased level of expression of positive product area (P<0.05), and a not significantly different grey level of posi-tive cells (P>0.05) although it decreased. The said findings indicated the followings: voluntary wheeling exercise can enhance the learning and memorizing ability of depressive rats;its mechanism may be related to that long-term voluntary wheeling exercise decreases the level of blood serum cortisol of depressive rats and enhances the c-fos expression of basolateral amygdaloid nucleus of their brains.