实用肝脏病杂志
實用肝髒病雜誌
실용간장병잡지
JOURNAL OF CLINICAL HEPATOLOGY
2013年
5期
448-450
,共3页
毛静怡%李鑫%王晨%徐有青
毛靜怡%李鑫%王晨%徐有青
모정이%리흠%왕신%서유청
急性肝损伤%肝脏贮备功能%吲哚氰绿%D-氨基半乳糖%大鼠
急性肝損傷%肝髒貯備功能%吲哚氰綠%D-氨基半乳糖%大鼠
급성간손상%간장저비공능%신타청록%D-안기반유당%대서
Acute liver injury%Liver function reserve%Indocyanine green%D-galnactosamine%Rats
目的探讨吲哚氰绿（indocyanine green，ICG）排泄试验在急性肝损伤肝功能评估中的价值。方法将32只SD大鼠随机分为4组，腹腔注射生理盐水或不同剂量D-氨基半乳糖（200mg.kg-1、700mg.kg-1或1200mg. kg-1）诱导急性肝损伤,于造模后24h测定吲哚氰绿15分钟滞留率（ICGR15）、肝脏病理学积分及两者的相关性。另将48只SD大鼠随机分为6组，腹腔注射450mg.kg-1D-氨基半乳糖，于0h和造模后6h、12h、24h、48h和72h分别测定ICGR15、肝脏病理学积分及两者的相关性。结果对照组和不同浓度D-氨基半乳糖组病理学积分分别为2.71±0.04、18.40±5.05、79.71±16.98和102.50±18.69分，ICGR15分别为3.8±0.5%、4.0±0.6%、17.3±4.3%和21.5±3.4%，ICGR15与肝脏病理学评分呈显著正相关（r=0.7102，P<0.001）；在D-氨基半乳糖（450mg.kg-1）处理前（0h）和处理后6～72h肝脏病理学积分分别为0.40±0.07、2.86±1.85、5.90±0.95、8.01±0.43、3.66±0.34和2.17±0.40分，其中处理后6h和72h组病理学积分显著高于处理前（P<0.05），而12h、24h和48h组病理学积分显著高于6h组（P<0.01）。ICGR15分别为4.8±0.6%、6.1±1.2%、6.9±1.6%、17.0±1.3%、12.5±7.3%和5.6±2.8%，其中12h组、24h组和48h组ICGR15显著高于6h组（P<0.05）；不同时间处理组ICGR15与肝组织病理学积分也呈正相关（r=0.6970，P<0.001）。结论 ICGR15可以动态和准确地反映急性肝损伤时肝脏的贮备功能。
目的探討吲哚氰綠（indocyanine green，ICG）排洩試驗在急性肝損傷肝功能評估中的價值。方法將32隻SD大鼠隨機分為4組，腹腔註射生理鹽水或不同劑量D-氨基半乳糖（200mg.kg-1、700mg.kg-1或1200mg. kg-1）誘導急性肝損傷,于造模後24h測定吲哚氰綠15分鐘滯留率（ICGR15）、肝髒病理學積分及兩者的相關性。另將48隻SD大鼠隨機分為6組，腹腔註射450mg.kg-1D-氨基半乳糖，于0h和造模後6h、12h、24h、48h和72h分彆測定ICGR15、肝髒病理學積分及兩者的相關性。結果對照組和不同濃度D-氨基半乳糖組病理學積分分彆為2.71±0.04、18.40±5.05、79.71±16.98和102.50±18.69分，ICGR15分彆為3.8±0.5%、4.0±0.6%、17.3±4.3%和21.5±3.4%，ICGR15與肝髒病理學評分呈顯著正相關（r=0.7102，P<0.001）；在D-氨基半乳糖（450mg.kg-1）處理前（0h）和處理後6～72h肝髒病理學積分分彆為0.40±0.07、2.86±1.85、5.90±0.95、8.01±0.43、3.66±0.34和2.17±0.40分，其中處理後6h和72h組病理學積分顯著高于處理前（P<0.05），而12h、24h和48h組病理學積分顯著高于6h組（P<0.01）。ICGR15分彆為4.8±0.6%、6.1±1.2%、6.9±1.6%、17.0±1.3%、12.5±7.3%和5.6±2.8%，其中12h組、24h組和48h組ICGR15顯著高于6h組（P<0.05）；不同時間處理組ICGR15與肝組織病理學積分也呈正相關（r=0.6970，P<0.001）。結論 ICGR15可以動態和準確地反映急性肝損傷時肝髒的貯備功能。
목적탐토신타청록（indocyanine green，ICG）배설시험재급성간손상간공능평고중적개치。방법장32지SD대서수궤분위4조，복강주사생리염수혹불동제량D-안기반유당（200mg.kg-1、700mg.kg-1혹1200mg. kg-1）유도급성간손상,우조모후24h측정신타청록15분종체류솔（ICGR15）、간장병이학적분급량자적상관성。령장48지SD대서수궤분위6조，복강주사450mg.kg-1D-안기반유당，우0h화조모후6h、12h、24h、48h화72h분별측정ICGR15、간장병이학적분급량자적상관성。결과대조조화불동농도D-안기반유당조병이학적분분별위2.71±0.04、18.40±5.05、79.71±16.98화102.50±18.69분，ICGR15분별위3.8±0.5%、4.0±0.6%、17.3±4.3%화21.5±3.4%，ICGR15여간장병이학평분정현저정상관（r=0.7102，P<0.001）；재D-안기반유당（450mg.kg-1）처리전（0h）화처리후6～72h간장병이학적분분별위0.40±0.07、2.86±1.85、5.90±0.95、8.01±0.43、3.66±0.34화2.17±0.40분，기중처리후6h화72h조병이학적분현저고우처리전（P<0.05），이12h、24h화48h조병이학적분현저고우6h조（P<0.01）。ICGR15분별위4.8±0.6%、6.1±1.2%、6.9±1.6%、17.0±1.3%、12.5±7.3%화5.6±2.8%，기중12h조、24h조화48h조ICGR15현저고우6h조（P<0.05）；불동시간처리조ICGR15여간조직병이학적분야정정상관（r=0.6970，P<0.001）。결론 ICGR15가이동태화준학지반영급성간손상시간장적저비공능。
Objective To evaluate the liver function reserve by indocyanine green (ICG) clearance in rats with acute liver injury. Methods Thirty-two male SD rats were randomly divided into four groups. The rats re-ceived saline or different doses of D-galnactosamine (200mg.kg-1,700mg.kg-1 and 1200mg.kg-1) injected intraperi-toneally to induce acute liver injury. ICG retention rates at 15min (ICGR15)and histopathological scores were ob-tained and the correlation between these two parameters was calculated 24 hrs after injection. Forty-eight male SD rats in the second experiment were randomly divided into six groups and received D-galnactosamine at dose of 450 mg.kg-1 injected intraperitoneally at six hour interval for 72h. ICGR15 and histopathological scores were obtained and the correlation between these two parameters was calculated. Results The histopathological scores of liver tissues in the controls or in the three models were 2.71±0.04,18.40±5.05,79.71±16.98 and 102.50±18.69, respectively and the ICGR15 were 3.8±0.5%,4.0±0.6%,17.3±4.3% and 21.5±3.4%,respectively;The histopathologi-cal scores were positively correlated with ICGR15 (r=0.7102,P<0.001);The histopathological scores of liver tissues before(0h)or after(6,12,24,48,72h)D-galnactosamine injection were 0.40±0.07,2.86±1.85,5.90±0.95,8.01±0.43, 3.66±0.34 and 2.17±0.40,respectively;The scores at 6h and 72h were significantly higher than that at baseline (0h,P<0.05),while scores at 12h,24h and 48h were significantly higher than that at 6h;The ICGR15 at different time points were 4.8±0.6%,6.1±1.2%,6.9±1.6%,17.0±1.3%,12.5±7.3% and 5.6±2.8%,respectively,and the ICGR15 at 12h,24h and 48h were significantly higher than that at 6h;The histopathological scores was also positively correlated with ICGR15 (r=0.6970,P<0.001). Conclusion ICG clearance test is a dynamic and effective indicator of liver functional reserve in rats with acute liver injury.
