CAJ | 학술논문

目的探讨乙型肝炎病毒感染者肝组织白细胞介素12（IL-12）表达与临床表现和肝脏病理学变化的关系。方法应用免疫组化法检测9例正常肝脏组织、183例慢性乙型肝炎（CHB）、41例乙型肝炎肝硬化、37例HBV相关肝癌患者肝组织IL-12表达情况，并与临床特点和病理分级等进行相关性分析。结果 CHB患者肝组织IL-12以弱阳性表达为主，阳性和强阳性表达的比例仅为26.8%，而肝硬化和肝癌患者阳性和强阳性表达的比例分别为36.6%和48.6%，均显著高于CHB患者（P＜0.05）；HBeAg阴转且HBV DNA阴转的CHB患者肝内IL-12阳性表达比例为64.3%，显著高于HBeAg阳性伴肝功能异常者（30.0%）和肝功能正常者（20.4%）（P＜0.05）；未发现IL-12表达与肝脏炎症分级或纤维化分期具有相关性。结论 IL-12可能参与HBV感染后的疾病进展与转归。
목적탐토을형간염병독감염자간조직백세포개소12（IL-12）표체여림상표현화간장병이학변화적관계。방법응용면역조화법검측9례정상간장조직、183례만성을형간염（CHB）、41례을형간염간경화、37례HBV상관간암환자간조직IL-12표체정황，병여림상특점화병리분급등진행상관성분석。결과 CHB환자간조직IL-12이약양성표체위주，양성화강양성표체적비례부위26.8%，이간경화화간암환자양성화강양성표체적비례분별위36.6%화48.6%，균현저고우CHB환자（P＜0.05）；HBeAg음전차HBV DNA음전적CHB환자간내IL-12양성표체비례위64.3%，현저고우HBeAg양성반간공능이상자（30.0%）화간공능정상자（20.4%）（P＜0.05）；미발현IL-12표체여간장염증분급혹섬유화분기구유상관성。결론 IL-12가능삼여HBV감염후적질병진전여전귀。
Objective To investigate the relationship between interleukin-12(IL-12) expression in liver tis-sues and the clinical significance in patients with hepatitis B virus infection-associated liver diseases. Methods IL-12 expression in the liver tissues of 183 patients with CHB,41 patients with liver cirrhosis and 37 patients with HCC was detected by immunohistochemical staining; The correlation of IL-12 expression to clinical features and pathological grading and staging was analyzed. Results IL-12 expression was mild positive in liver tissues from CHB patients,but was much stronger in cirrhotic and HCC patients;The positive and strong positive rates of IL-12 expression in patients with cirrhosis and with HCC were 36.6% and 48.6%,respectively,significantly higher than that in CHB patients (26.8%,P<0.05);The positive rate of IL-12 in CHB patients with HBeAg negative and HBV DNA loss was 64.3%,significantly higher than that in CHB patients with HbeAg positive and HBV DNA positive (30.0%,P<0.05);There was no correlation between the expression of IL-12 and liver inflammation grading and fi-brosis staging. Conclusions IL-12 may be involved in the disease progression and outcome of patients with chronic hepatitis B viral infection.