CAJ | 학술논문

石参为百合科独尾草属独尾草（ Eremurus chinensis Fedtsch ．）肉质根加工的稀有山野菜，主产于秦岭南坡局地。通过单因素和正交试验，研究了苯酚-硫酸法测定石参多糖含量时各因素对结果的影响，并对这些因素进行了优化和方法学验证。研究结果表明，苯酚用量1．6 mL、浓硫酸用量7．0 mL、显色时间30 min、显色温度80℃为苯酚-硫酸法测定石参多糖的最佳工艺条件，在此条件下平均回收率为98．64％，相对标准偏差（ RSD ）为1．40％；优化后的苯酚-硫酸法测定石参多糖准确性和稳定性高。比较分析表明，不同植物多糖的苯酚-硫酸法检测工艺有一定的差异，浓硫酸的加入方式对测定结果有较大影响。
석삼위백합과독미초속독미초（ Eremurus chinensis Fedtsch ．）육질근가공적희유산야채，주산우진령남파국지。통과단인소화정교시험，연구료분분-류산법측정석삼다당함량시각인소대결과적영향，병대저사인소진행료우화화방법학험증。연구결과표명，분분용량1．6 mL、농류산용량7．0 mL、현색시간30 min、현색온도80℃위분분-류산법측정석삼다당적최가공예조건，재차조건하평균회수솔위98．64％，상대표준편차（ RSD ）위1．40％；우화후적분분-류산법측정석삼다당준학성화은정성고。비교분석표명，불동식물다당적분분-류산법검측공예유일정적차이，농류산적가입방식대측정결과유교대영향。
Shishen , a rare edible wild herb produced in Qinling Mountains , is the process fleshy roots from Eremurus chinensis belonging to genus of Eremurus, Liliaceae.Through the single factor experiments and orthogonal experiments , the factors affecting the determination of polysaccharide content in Shishen ( E. chinensis) with phenol-sulfuric acid method were optimized .The results showed that the optimum determina-tion conditions were 1.6 mL phenol, 7.0 mL sulphuric acid, 80 ℃reaction temperature and 30 min reaction time.The average recovery was up to 98.64%, and the RSD was 1.40%.The comparative analysis indicated that the way used with phenol-sulfuric acid and how to add sulphuric acid maybe result in a great of influence to the results for different polysaccharides test .In addition , the modified phenol-sulfuric acid method to deter-minate polysaccharides is accurate and steady .