生物技术进展
生物技術進展
생물기술진전
2013年
5期
363-367
,共5页
植生拉乌尔菌%碳青霉烯酶%质粒
植生拉烏爾菌%碳青黴烯酶%質粒
식생랍오이균%탄청매희매%질립
Raoultella planticola%carbapenemase%plasmid
碳青霉烯酶编码基因的传播是临床抗菌治疗面临的严重威胁之一。采用PCR、Southern杂交、测序比对等方法筛查和研究了碳青霉烯类抗菌药物的相关耐药基因。首次在国内发现了产KPC酸的植生拉乌尔菌两株。分离到的植生拉乌尔菌对被测试的19种常用抗菌药物中的16种耐药，只对多粘菌素B、粘菌素和米诺环素敏感。它们均产KPC-2型碳青霉烯酶，其编码基因blaKPC-2位于质粒上，两侧（包括blaKPC-2）长度为7498 bp的DNA序列所包含的结构从上游至下游分别为Tn3-transposase， Tn3-resolvase， ISKpn8， blaKPC-2和ISKpn6-like元件。
碳青黴烯酶編碼基因的傳播是臨床抗菌治療麵臨的嚴重威脅之一。採用PCR、Southern雜交、測序比對等方法篩查和研究瞭碳青黴烯類抗菌藥物的相關耐藥基因。首次在國內髮現瞭產KPC痠的植生拉烏爾菌兩株。分離到的植生拉烏爾菌對被測試的19種常用抗菌藥物中的16種耐藥，隻對多粘菌素B、粘菌素和米諾環素敏感。它們均產KPC-2型碳青黴烯酶，其編碼基因blaKPC-2位于質粒上，兩側（包括blaKPC-2）長度為7498 bp的DNA序列所包含的結構從上遊至下遊分彆為Tn3-transposase， Tn3-resolvase， ISKpn8， blaKPC-2和ISKpn6-like元件。
탄청매희매편마기인적전파시림상항균치료면림적엄중위협지일。채용PCR、Southern잡교、측서비대등방법사사화연구료탄청매희류항균약물적상관내약기인。수차재국내발현료산KPC산적식생랍오이균량주。분리도적식생랍오이균대피측시적19충상용항균약물중적16충내약，지대다점균소B、점균소화미낙배소민감。타문균산KPC-2형탄청매희매，기편마기인blaKPC-2위우질립상，량측（포괄blaKPC-2）장도위7498 bp적DNA서렬소포함적결구종상유지하유분별위Tn3-transposase， Tn3-resolvase， ISKpn8， blaKPC-2화ISKpn6-like원건。
The increasing emergence and spread of Klebsiella pneumoniae carbapenemases ( KPCs ) leaves fewer available therapeutic options due to their broad-spectrum hydrolytic activity. Two KPC-2-producing Raoultella planticola isolates were first identified and analyzed using PCR, Southern hybridization and sequencing in China. Two isolates had an identical antimicrobial susceptibility profile, blaKPC-2 gene as well as its flanking sequences. They were resistant to 16 antimicrobial agents while sensitive to polymyxin B, colistin and minocycline. The results demonstrated that the blaKPC-2 gene was located on a 7 498 bp plasmid segment that encoded five open reading frames with the order of Tn3-transposase, Tn3-resolvase, ISKpn8, blaKPC-2 gene and ISKpn6-like.
