CAJ | 학술논문

采用组织化学和原子吸收分析等方法，研究了中华绒螯蟹蜕皮过程中体壁结构和主要成分的变化。结果显示：中华绒螯蟹体壁分为上表皮、外表皮、内表皮和膜层，糖类物质各层均有分布，胶原纤维分布在除上表皮外的其他各层。在蜕皮前，糖类、胶原纤维都被重吸收，体壁上表皮和外表皮在蜕皮前形成，内表皮和膜层在蜕皮后形成。体壁粗蛋白含量在蜕皮前期(D1-D3-4期)降低(P<0.05)，蜕皮后 A-B 期含量极高(P<0.05)。几丁质含量在蜕皮过程中变化不显著(P>0.05)，只是在蜕皮前稍有上升。Ca2+和 Mg2+含量在蜕皮前D1期显著低于蜕皮间期和蜕皮前其他时期(P<0.05)，而蜕皮后A-B期降到最低(P<0.05)，蜕下的甲壳中则含有较多的 Ca2+和 Mg2+(P<0.05)。Cu2+和 Zn2+含量除蜕皮后 A-B 期升高外(P<0.05)，其余时期变化不明显(P>0.05)。这些研究结果表明，中华绒螯蟹体壁结构和成分变化与蜕皮周期密切相关。
채용조직화학화원자흡수분석등방법，연구료중화융오해세피과정중체벽결구화주요성분적변화。결과현시：중화융오해체벽분위상표피、외표피、내표피화막층，당류물질각층균유분포，효원섬유분포재제상표피외적기타각층。재세피전，당류、효원섬유도피중흡수，체벽상표피화외표피재세피전형성，내표피화막층재세피후형성。체벽조단백함량재세피전기(D1-D3-4기)강저(P<0.05)，세피후 A-B 기함량겁고(P<0.05)。궤정질함량재세피과정중변화불현저(P>0.05)，지시재세피전초유상승。Ca2+화 Mg2+함량재세피전D1기현저저우세피간기화세피전기타시기(P<0.05)，이세피후A-B기강도최저(P<0.05)，세하적갑각중칙함유교다적 Ca2+화 Mg2+(P<0.05)。Cu2+화 Zn2+함량제세피후 A-B 기승고외(P<0.05)，기여시기변화불명현(P>0.05)。저사연구결과표명，중화융오해체벽결구화성분변화여세피주기밀절상관。
The construction and main constituent of the integument of Eriocheir sinensis were studied by the methods of histochemistry and atomic absorption methods. The results showed that the integument of E. sinensis was composed of four sub-layers from the exterior to the interior:an epicuticle, an exocuticle, an endocuticle, and a membranous layer. The carbohydrates were deposited in all sub-layers and the collagen fibers were deposited in all sub-layers except the epicu-ticle. Both carbohydrates and collagen fibers were reabsorbed in premolt stages. The epicuticle and exocuticle were synthesized before molt and the endocuticle and membranous layer were synthesized after molt. The contents of in-tegumental crude protein decreased before molt (D1-D3-4) (P<0.05) and were extremely high in postmolt A-B stage (P<0.05). The integumental chitinous content showed no obvious variation during the molt cycle (P>0.05) except a little increase before molt. The contents of Ca2+and Mg2+in premolt D1 stage were significantly lower compared with the in-termolt stage and other stages in premolt (P<0.05) and were the lowest in the postmolt A-B stage (P<0.05). There were much Ca2+and Mg2+in the exoskeleton that the animal moulted. The content of Cu2+and Zn2+showed no obvious varia-tion except increased in postmolt A-B stage (P<0.05). These results indicated that the structural and constituent changes in integument of E. sinensis are coincident with its molt stages.