CAJ | 학술논문

运用 PCR-SSCP 技术研究100尾牙鲆(Paralichthys olivaceus)MyoD 基因的单核苷酸多态性(Single nucleotide polymorphisms, SNPs),并将筛选到的突变位点与牙鲆生长性状进行相关性分析。结果表明,在外显子1和内含子1上存在3个SNPs,在外显子1(MyoD2)基因座发现3种基因型AA、AB和BB, G863A突变,属于同义突变。在内含子 MyoD4基因座检测到 DD、FF、CD、CE、DE 和 DF 型个体。利用最小二乘法研究MyoD基因多态性位点对牙鲆生长性状的影响。结果表明,外显子1的SNPs对生长性状无显著影响(P>0.05)。内含子1的SNPs对牙鲆的生长性状影响均显著(P<0.05)。研究结果为SNPs位点与牙鲆生长性能关联分析奠定了基础。
운용 PCR-SSCP 기술연구100미아평(Paralichthys olivaceus)MyoD 기인적단핵감산다태성(Single nucleotide polymorphisms, SNPs),병장사선도적돌변위점여아평생장성상진행상관성분석。결과표명,재외현자1화내함자1상존재3개SNPs,재외현자1(MyoD2)기인좌발현3충기인형AA、AB화BB, G863A돌변,속우동의돌변。재내함자 MyoD4기인좌검측도 DD、FF、CD、CE、DE 화 DF 형개체。이용최소이승법연구MyoD기인다태성위점대아평생장성상적영향。결과표명,외현자1적SNPs대생장성상무현저영향(P>0.05)。내함자1적SNPs대아평적생장성상영향균현저(P<0.05)。연구결과위SNPs위점여아평생장성능관련분석전정료기출。
Single nucleotide polymorphisms (SNPs) of MyoD gene were analyzed in 100 Japanese flounder with PCR-SSCP Association between the polymorphisms and growth traits were analyzed. The results showed that the poly-morphism sites were identified in exon 1(MyoD2) and intron 1(MyoD4). Three genotypes were found at the MyoD2 locus, denoted AA, AB and BB, and polymorphism site was G863A, being synonymous mutation. DD, FF, CD, CE, DE and DF genotypes were found at the MyoD4 locus. The association between different genotypes and Japanese flounder growth traits were analyzed by leastsquare analysis. The results indicated that there was no significant effect in the SNPs of exon 1 on growth traits (P>0.05), the SNPs in intron 1 were significantly associated with the body weight, body length and body height (P<0.05). These SNPs might be useful for a further research on MyoD genes relation to produc-tion performance in Japanese flounder.