中国药物经济学
中國藥物經濟學
중국약물경제학
CHINA JOURNAL OF PHARMACEUTICAL ECONOMICS
2013年
6期
27-28
,共2页
三七总皂苷%ICAM-1%痛风
三七總皂苷%ICAM-1%痛風
삼칠총조감%ICAM-1%통풍
Total saponins three seven%ICAM-1%Gout
目的本文以尿酸钠100μg?mL-1诱导体外急性炎症模型，观察三七总皂苷对 ICAM-1表达及内皮细胞凋亡的影响，探讨临床用于痛风的价值。方法采用2.5、5.0、10.0μg?mL-1浓度的三七总皂苷进行预干预，实时定量PCR检测ICAM-1的基因表达，流式细胞仪测定细胞凋亡率。结果三七总皂苷组2.5、5.0、10.0μg?mL-1明显抑制细胞凋亡（6.90±1.07）%，与模型组比较 P＜0.01；能显著抑制 ICAM-1基因的表达。结论三七总皂苷显著抑制炎症因子 ICAM-1的表达及细胞凋亡。
目的本文以尿痠鈉100μg?mL-1誘導體外急性炎癥模型，觀察三七總皂苷對 ICAM-1錶達及內皮細胞凋亡的影響，探討臨床用于痛風的價值。方法採用2.5、5.0、10.0μg?mL-1濃度的三七總皂苷進行預榦預，實時定量PCR檢測ICAM-1的基因錶達，流式細胞儀測定細胞凋亡率。結果三七總皂苷組2.5、5.0、10.0μg?mL-1明顯抑製細胞凋亡（6.90±1.07）%，與模型組比較 P＜0.01；能顯著抑製 ICAM-1基因的錶達。結論三七總皂苷顯著抑製炎癥因子 ICAM-1的錶達及細胞凋亡。
목적본문이뇨산납100μg?mL-1유도체외급성염증모형，관찰삼칠총조감대 ICAM-1표체급내피세포조망적영향，탐토림상용우통풍적개치。방법채용2.5、5.0、10.0μg?mL-1농도적삼칠총조감진행예간예，실시정량PCR검측ICAM-1적기인표체，류식세포의측정세포조망솔。결과삼칠총조감조2.5、5.0、10.0μg?mL-1명현억제세포조망（6.90±1.07）%，여모형조비교 P＜0.01；능현저억제 ICAM-1기인적표체。결론삼칠총조감현저억제염증인자 ICAM-1적표체급세포조망。
Objective In this paper, uric acid sodium 100μg?mL-1 induced acute inflammation model in vitro, to observe the effect of total saponins of three seven on ICAM-1 expression and apoptosis of endothelial cells, to investigate the clinical value of for ventilation.Methods The total saponins of three seven 2.5,5.0,10.0μg?mL-1 concentration of pre intervention, expression of real-time quantitative PCR detection of ICAM-1 gene,cellapoptosis was detected by flow cytometry. Results Total saponins of three seven groups of 2.5,5.0,10.0μg?mL-1 significantly inhibited apoptosis (6.90±1.07)%, compared with model group, P<0.01;expression can significantly inhibit ICAM-1 gene.Conclusion Total saponins three seven inhibited the cellapoptosis and expression of ICAM-1 cytokines.
