CAJ | 학술논문

目的建立紫外-可见分光光度法测定不同产地山楂果实中总黄酮含量。方法采用超声法提取山楂果实中的总黄酮，采用紫外-可见分光光度法测定不同产地山楂总黄酮含量，检测波长为500nm。结果芦丁在质量浓度20～120μg/mL（r=0.9993）范围内与吸光度值线性关系良好，平均回收率为100.1%，RSD=0.3%（n=6），来自8个产地的总黄酮含量在3.53%～4.12%之间。结论本方法简便、准确，重复性好，可快速测定山楂果实中总黄酮的含量；不同产地山楂总黄酮的含量存在一定差异，本实验可为控制山楂的质量提供参考依据。
목적건립자외-가견분광광도법측정불동산지산사과실중총황동함량。방법채용초성법제취산사과실중적총황동，채용자외-가견분광광도법측정불동산지산사총황동함량，검측파장위500nm。결과호정재질량농도20～120μg/mL（r=0.9993）범위내여흡광도치선성관계량호，평균회수솔위100.1%，RSD=0.3%（n=6），래자8개산지적총황동함량재3.53%～4.12%지간。결론본방법간편、준학，중복성호，가쾌속측정산사과실중총황동적함량；불동산지산사총황동적함량존재일정차이，본실험가위공제산사적질량제공삼고의거。
Objective To establish a UV-VIS spectrophotometry method to determine total flavonoids in Crataegus pinnatifida Bge. from different areas. Methods Total flavonoids of Crataegus pinnatifida Bge. was extracted by ultrasonic method, and was determined by UV-VIS spectrophotometry at 500 nm. Results The linearity of rutin was20~120μg/mL(r=0.9993), and the average recovery ratios was 100.1%. The RSD was 0.3%(n=6). The contents of total flavonoids ranged from 3.53%to 4.12%. Conclusion The method was simple and accurate, and it could be used for the determination of total flavonoids quickly. The contents of total flavonoids was different from different areas.It provided some academic basis for quality standard of Crataegus pinnatifida Bge..